Watkins W B, Moore R Y
Am J Anat. 1980 Aug;158(4):445-54. doi: 10.1002/aja.1001580406.
The distribution of those cells in the anterior pituitary gland of the rat which stain immunohistochemically with rabbit anti-human calcitonin serum has been examined. Immunoreactive cells were confined primarily to the ventral surface of the gland and possessed both a distribution and morphology distinct from the corticotrophs. Staining of serial thin sections with rabbit anti-rat TSH-subunit serum resulted in an immunoreaction in those cells that stained for calcitonin. However, not all the thyrotrophs gave a positive immunoreaction for calcitonin. It is concluded from this study that it is inappropriate to attribute calcitonin as being part of the 31K-dalton precursor for adrenocorticotropin, a hypothesis that was proposed earlier. In the immunohistochemical reaction with anti-calcitonin serum, it was found that relatively high concentrations of antigen (500 micrograms/ml) were required in absorption experiments in order to inhibit staining. Furthermore, the staining of thyrotrophs with the anti-calcitonin serum was inhibited after preadsorption of the primary antiserum with excess rat beta-TSH (1000 micrograms/ml). Because of these immunochemical characteristics, it is questionable whether the calcitonin-like material observed in the rat pituitary gland is chemically identical to that of thyroidal calcitonin.
已对大鼠垂体前叶中用兔抗人降钙素血清进行免疫组织化学染色的细胞分布进行了研究。免疫反应性细胞主要局限于腺的腹面，其分布和形态与促肾上腺皮质激素细胞均不同。用兔抗大鼠促甲状腺激素亚基血清对连续薄切片进行染色，结果显示在那些对降钙素染色的细胞中有免疫反应。然而，并非所有促甲状腺激素细胞对降钙素都呈阳性免疫反应。从这项研究得出的结论是，将降钙素归因于促肾上腺皮质激素31K道尔顿前体的一部分是不合适的，这是先前提出的一个假说。在与抗降钙素血清的免疫组织化学反应中，发现在吸收实验中需要相对高浓度的抗原（500微克/毫升）才能抑制染色。此外，在用过量大鼠β-促甲状腺激素（1000微克/毫升）预吸附一抗血清后用抗降钙素血清对促甲状腺激素细胞的染色受到抑制。由于这些免疫化学特性，在大鼠垂体中观察到的降钙素样物质在化学上是否与甲状腺降钙素相同值得怀疑。
