一种从大鼠和兔主动脉制备内皮（内皮细胞）单层标本的简单快速方法。
A simple rapid method for the preparation of en face endothelial (Häutchen) monolayers from rat and rabbit aortas.
作者信息
Hirsch E Z, Martino W, Orr C H, White H, Chisolm G M
出版信息
Atherosclerosis. 1980 Dec;37(4):539-48. doi: 10.1016/0021-9150(80)90061-1.
This report describes a new method for preparing Häutchen monolayers for autoradiography with photographic emulsion contacting either the abluminal or luminal endothelial surface. Included is a means for monitoring completeness of the monolayer and retrieving cells which fail to adhere to it. Cell retrieval may be important in studies requiring autographic evaluation of endothelial cell populations. The procedure has been applied to endothelium containing newly reendothelialized aortic regions.
本报告描述了一种制备用于放射自显影的内皮细胞单层的新方法，其通过使摄影乳剂接触管腔外或管腔内的内皮表面来实现。该方法包括一种监测单层完整性以及回收未能附着于其上的细胞的手段。在需要对内皮细胞群体进行放射自显影评估的研究中，回收细胞可能很重要。该程序已应用于含有新内皮化主动脉区域的内皮细胞。
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