通过形成聚集体分子将增强因子转化为抑制因子。
The conversion of an enhancing factor to a suppressor factor by the formation of an aggregate molecule.
作者信息
Mulcahy H L, Rubin A S, MacDonald A B
出版信息
Immunology. 1981 Jan;42(1):37-43.
A non-specific suppressor factor, owl monkey suppressor factor, OMSF, from leukaemic owl monkey peripheral T lymphocytes has been isolated and characterized. OMSF is a glycoprotein of a molecular weight of 66,000 which can be produced from purified owl monkey enhancing factor, OMEF, by both Tris buffer dialysis and by repeated freezing and thawing. SDS gel electrophoresis demonstrates that OMSF is composed of 39,000 mol. wt subunits which co-migrate with the OMEF molecule. This suppressor acts at less than nanogram levels on day 0 of the in vitro immune response to suppress the number of plaque-forming cells produced to sheep red blood cells in a murine assay system and has little effect when added on day 2 to this system. OMSF is bound by antibodies raised specifically against OMEF and inhibits the binding of OMEF to this antiserum in a competitive radioimmunoassay. These results suggest that both enhancement and suppression of the immune response can be mediated by the same molecule under different circumstances.
一种来自白血病夜猴外周血T淋巴细胞的非特异性抑制因子，即夜猴抑制因子（OMSF）已被分离并鉴定。OMSF是一种分子量为66,000的糖蛋白，通过Tris缓冲液透析和反复冻融，可从纯化的夜猴增强因子（OMEF）产生。SDS凝胶电泳表明，OMSF由分子量为39,000的亚基组成，这些亚基与OMEF分子共同迁移。这种抑制因子在体外免疫反应的第0天以低于纳克的水平起作用，在小鼠检测系统中抑制针对绵羊红细胞产生的噬斑形成细胞数量，而在第2天添加到该系统时几乎没有作用。OMSF能被针对OMEF特异性产生的抗体结合，并在竞争性放射免疫分析中抑制OMEF与该抗血清的结合。这些结果表明，在不同情况下，免疫反应的增强和抑制可由同一分子介导。
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