Uchańska-Ziegler B, Wernet P, Ziegler A
J Immunol Methods. 1980;39(1-2):85-93. doi: 10.1016/0022-1759(80)90297-5.
A method is described which allows incubation, washing and staining of cells for immunofluorescence analysis to be carried out in microtiter plates. Comparison of this procedure with the conventional protocol, carried out on normal and leukemic human peripheral blood cells, reveals 5 major advantages. (1) Only 2.5 X 10(5) viable cells are needed for testing a particular antiserum. (2) The amount of reagents needed is 1/4 of that used in the conventional method. (3) Damage to cells is reduced to a minimum by shorter processing times and gentler centrifugation steps. (4) A large number of samples can be processed at the same time in an identical and reproducible manner. (5) The cost of an experiment is considerable reduced.
本文描述了一种方法，该方法可在微量滴定板中进行细胞的孵育、洗涤和染色，用于免疫荧光分析。将此程序与常规方案进行比较，对正常和白血病患者外周血细胞进行操作，发现有5个主要优点。（1）检测一种特定抗血清仅需2.5×10⁵个活细胞。（2）所需试剂用量是传统方法的1/4。（3）通过缩短处理时间和采用更温和的离心步骤，将对细胞的损伤降至最低。（4）可以以相同且可重复的方式同时处理大量样本。（5）实验成本大幅降低。
