Chang T H, Steplewski Z, Koprowski H
J Immunol Methods. 1980;39(4):369-75. doi: 10.1016/0022-1759(80)90237-9.
We have succeeded in growing several established hybridoma cell lines (1116NS-19; 116NS-33a; 1116NS-52a; 1083-17-1A; 691-19-19) in a serum free medium supplemented with physiological concentrations of insulin (5 microgram/ml) and transferrin (5 microgram/ml). The hybridoma cells replicate in this medium and will secrete monoclonal antibodies in quantities comparable to those produced in the presence of serum. Adapted hybridoma cultures have been secreting antibodies in the supplemented serum free (SSF) medium for more than 3 months. Parental mouse myeloma P3x63Ag8 cells and non-secreting subclone 653 cells cannot survive longer than 6 days under the same conditions. Both insulin and transferrin are necessary for hybridoma cells to grow in serum free medium and omission of either one will not permit cell multiplication.
我们已成功在补充了生理浓度胰岛素（5微克/毫升）和转铁蛋白（5微克/毫升）的无血清培养基中培养了几种成熟的杂交瘤细胞系（1116NS - 19；116NS - 33a；1116NS - 52a；1083 - 17 - 1A；691 - 19 - 19）。杂交瘤细胞在这种培养基中能复制，并分泌与在有血清存在时产生的量相当的单克隆抗体。适应后的杂交瘤培养物已在补充了血清的无血清（SSF）培养基中分泌抗体超过3个月。亲本小鼠骨髓瘤P3x63Ag8细胞和不分泌的亚克隆653细胞在相同条件下存活时间不超过6天。胰岛素和转铁蛋白对于杂交瘤细胞在无血清培养基中生长都是必需的，缺少任何一种都不能使细胞增殖。
