Chen Z, Ke Y, Chen Y
Research Institute of Biochemical Engineering, East China University of Chemical Technology, Shanghai.
Cytotechnology. 1993;11(3):169-74. doi: 10.1007/BF00749866.
The effects of several different substances, including insulin, transferrin, ethanolamine, selenite and butyrate on the growth of murine hybridoma 2F7 cells, which secrete monoclonal antibody against small cell lung cancer, were investigated, and a serum-free medium SFM I was formulated. The effects of taurine, spermidine, progesterone and adenine on the cell growth were tested further on the basis of the medium SFM I, and a modified serum-free medium SFM II was established. On the basis of medium SFM II, the substitution tests of ferric citrate for transferrin were carried out, and it was found that transferrin could be replaced. The experiments suggested that the formulated serum-free medium was suitable for 2F7 cell growth and monoclonal antibody secretion, and thus facilitated subsequent purification of monoclonal antibody.
研究了包括胰岛素、转铁蛋白、乙醇胺、亚硒酸盐和丁酸盐在内的几种不同物质对分泌抗小细胞肺癌单克隆抗体的小鼠杂交瘤2F7细胞生长的影响，并配制了无血清培养基SFM I。在培养基SFM I的基础上，进一步测试了牛磺酸、亚精胺、孕酮和腺嘌呤对细胞生长的影响，并建立了改良的无血清培养基SFM II。在培养基SFM II的基础上，进行了柠檬酸铁替代转铁蛋白的替代试验，发现转铁蛋白可以被替代。实验表明，所配制的无血清培养基适合2F7细胞生长和单克隆抗体分泌，从而便于后续单克隆抗体的纯化。
