Buu N T, Duhaime J, Savard C, Truong L, Kuchel O
J Neurochem. 1981 Feb;36(2):769-72. doi: 10.1111/j.1471-4159.1981.tb01655.x.
A new method of measuring catecholamine (CA) sulfate permitted us to detect its presence in rat brain for the first time. The procedure consisted of separating the CA sulfate from the free CA by alumina adsorption followed by passage through Dowex, and measuring the CA sulfate by a radioenzymatic assay in the presence of a sulfatase. This method permitted demonstration of the presence of dopamine sulfate, and occasionally, of norepinephrine and epinephrine sulfate in the hypothalamus, striatum, and hippocampus of rat brain.
一种测量硫酸儿茶酚胺（CA）的新方法使我们首次能够在大鼠脑中检测到它的存在。该方法包括通过氧化铝吸附将硫酸CA与游离CA分离，然后通过Dowex柱，再在硫酸酯酶存在的情况下通过放射酶法测量硫酸CA。这种方法能够证明大鼠脑的下丘脑、纹状体和海马体中存在硫酸多巴胺，偶尔还能检测到硫酸去甲肾上腺素和硫酸肾上腺素。
