Haładus E, Zuk J
Mol Gen Genet. 1980;180(3):591-6. doi: 10.1007/BF00268065.
Spontaneous and UV-induced mitotic recombination was compared in diploids homozygous for rad6-1 mutation and in the wild-type strain carrying heterozygous markers for detecting gene conversion (hom2-1, hom2-2) and crossing over (ade1, ade2). Diploids homozygous for rad6-1 mutation were characterised by an elevated level of spontaneous and UV-induced mitotic recombination, particularly the intergenic events. Exposure of UV-irradiated strains to visible light resulted in an increased survival and decreased level of mitotic recombination. Liquid holding (LH) differentially affected frequency of mitotic intergenic and intragenic recombination in mutant and wild-type strains, being without any significant effect on cell survival. In a mutant strain intragenic recombination is significantly increased, intergenic only slightly. In the wild-type strain intragenic recombination is slightly decreased but intergenic is not changed by LH. Visible light applied after LH had no effect on survival and mitotic recombination in the wild type, while in the mutant strain photoreactivability of survival was fully preserved and accompanied by a decrease in the frequency of intragenic and intergenic recombination. The results suggest that metabolic pathways responsible for restoring cell survival are independent of or only partly overlapping with those concerning recombination events.
对携带rad6 - 1突变纯合子的二倍体以及携带用于检测基因转换（hom2 - 1、hom2 - 2）和交换（ade1、ade2）的杂合标记的野生型菌株中的自发和紫外线诱导的有丝分裂重组进行了比较。携带rad6 - 1突变纯合子的二倍体的特征在于自发和紫外线诱导的有丝分裂重组水平升高，特别是基因间事件。将紫外线照射的菌株暴露于可见光下会导致存活率增加和有丝分裂重组水平降低。液体保存（LH）对突变体和野生型菌株中有丝分裂基因间和基因内重组的频率有不同影响，对细胞存活率没有任何显著影响。在突变菌株中，基因内重组显著增加，基因间重组仅略有增加。在野生型菌株中，基因内重组略有减少，但基因间重组不受LH影响。在LH后施加可见光对野生型的存活率和有丝分裂重组没有影响，而在突变菌株中，存活率的光复活能力得到完全保留，并伴随着基因内和基因间重组频率的降低。结果表明，负责恢复细胞存活的代谢途径与那些涉及重组事件的途径独立或仅部分重叠。
