Wiker H G, Harboe M, Bennedsen J, Closs O
Institute of Immunology and Rheumatology, University of Oslo, Norway.
Scand J Immunol. 1988 Feb;27(2):223-39. doi: 10.1111/j.1365-3083.1988.tb02342.x.
On the basis of a previously developed reference system for Mycobacterium bovis, BCG, in crossed immunoelectrophoresis (CIE), antigens of Mycobacterium tuberculosis were identified in an analogous system. A majority of the numbered lines in the BCG system were also present and identified in the M. tuberculosis system. The corresponding antigens in the two systems were identified by dual dilution in CIE, and using monospecific antisera and monoclonal antibodies. Some of the antigens were specifically identified by the demonstration of enzyme activity and by means of hydroxyapatite, concanavalin A (Con A), EDTA, and blue-Sepharose. Three antigens (nos 10, 78, and 81), which were found in high concentrations in M. tuberculosis culture fluid, were not identified or were present in low concentrations in BCG culture fluid. The high percentage of corresponding antigens confirms that there is a very close taxonomic relationship between BCG and M. tuberculosis. Corresponding antigens in BCG and M. tuberculosis did not differ in electrophoretic mobility in the antigenic preparations studied.
基于先前建立的牛分枝杆菌卡介苗在交叉免疫电泳（CIE）中的参考系统，在类似系统中鉴定了结核分枝杆菌的抗原。卡介苗系统中大多数编号条带在结核分枝杆菌系统中也存在并被鉴定。通过CIE中的双重稀释、使用单特异性抗血清和单克隆抗体，确定了两个系统中的相应抗原。通过酶活性的证明以及借助羟基磷灰石、伴刀豆球蛋白A（Con A）、乙二胺四乙酸（EDTA）和蓝色琼脂糖，对一些抗原进行了特异性鉴定。在结核分枝杆菌培养液中高浓度发现的三种抗原（编号10、78和81），在卡介苗培养液中未被鉴定或浓度很低。相应抗原的高比例证实了卡介苗和结核分枝杆菌之间存在非常密切的分类学关系。在所研究的抗原制剂中，卡介苗和结核分枝杆菌的相应抗原在电泳迁移率上没有差异。
