Wüst J
Schweiz Med Wochenschr. 1980 Oct 25;110(43):1560-2.
To avoid false positive results it is very important that specimens for culture of anaerobic bacteria are not contaminated with normal flora from the skin or mucous membranes (mouth, intestinal tract, genital tract). The specimen should be transported to the laboratory as fast as possible without exposure to oxygen. Liquid specimens, such as aspirates, are superior to swabs. For rapid presumptive diagnosis suitable methods are gram stain and direct gas-liquid chromatography for fatty acids. Culture is laborious and time-consuming.
为避免出现假阳性结果,至关重要的是,用于培养厌氧菌的标本不能被来自皮肤或黏膜(口腔、肠道、生殖道)的正常菌群污染。标本应尽快运送至实验室,避免暴露于氧气中。液体标本,如吸出物,优于拭子。对于快速初步诊断,合适的方法是革兰氏染色和脂肪酸直接气-液色谱分析。培养操作繁琐且耗时。