Menozzi M G, Toni M, Schito G C
Ann Ist Super Sanita. 1979;15(1):155-65.
The isolation and identification of anaerobic bacteria by biochemical tests take long time, so it is very useful the gas-chromatography to detect the volatile fatty acids produced from their metabolism. This technique can give rapidly a presumptive diagnosis of anaerobic infection so that it is possible to start immediately a correct antibiotic therapy. In this study we have examined many samples of pus and exudate, and the results have been compared with those obtained from the culture. We have found a good correlation between the detection by gas-chromatography of isobutyric and butyric acids and the isolation in the culture of anaerobic flora, and particularly of Bacteroides fragilis when the gas-chromatography detected isobutyric, butyric and succinic acids.
通过生化试验分离和鉴定厌氧菌耗时较长,因此气相色谱法检测其代谢产生的挥发性脂肪酸非常有用。该技术可快速给出厌氧菌感染的初步诊断,从而能够立即开始正确的抗生素治疗。在本研究中,我们检测了许多脓液和渗出液样本,并将结果与培养所得结果进行了比较。我们发现,气相色谱法检测异丁酸和丁酸与培养中分离出厌氧菌,尤其是当气相色谱法检测到异丁酸、丁酸和琥珀酸时分离出脆弱拟杆菌之间存在良好的相关性。