Wüst J
Schweiz Med Wochenschr. 1980 Mar 8;110(10):362-9.
Current methods for isolation and identification of obligately anaerobic bacteria are laborious and time-consuming. Direct gas-liquid chromatography of purulent and serous specimens for short-chain fatty acids allows a presumptive diagnosis of anaerobic infection. Good correlation was found for the cultural recovery of anaerobic bacteria and the presence of propionic acid in concentrations of greater than or equal to 1 muMol/ml, isobutyric, butyric, isovaleric, valeric, isocaproic and caproic acid at greater than or equal to 0.1 muMol/ml, and succinic acid at greater than or equal to 2 muMol/ml (either as single acids or in combination). Specimens yielding no anaerobic bacteria in culture contained only acetic, lactic and small amounts of succinic acid, but none of the volatile acids.
目前用于分离和鉴定专性厌氧菌的方法既费力又耗时。对脓性和浆液性标本进行短链脂肪酸的直接气液色谱分析有助于对厌氧菌感染作出初步诊断。发现厌氧菌的培养回收率与浓度大于或等于1微摩尔/毫升的丙酸、大于或等于0.1微摩尔/毫升的异丁酸、丁酸、异戊酸、戊酸、异己酸和己酸以及大于或等于2微摩尔/毫升的琥珀酸（无论是单一酸还是组合形式）的存在之间具有良好的相关性。培养中未产生厌氧菌的标本仅含有乙酸、乳酸和少量琥珀酸，但不含挥发性酸。
