Wakizaka A, Okuhara E
Tohoku J Exp Med. 1977 Jun;122(2):161-6. doi: 10.1620/tjem.122.161.
The difference in the antigenic determinant size of DNA for sera from patients with SLE and rabbit anti-DNA sera were investigated. Haptenic inhibition studies were carried out by measuring the inhibition of [3H]DNA-antibody binding by three different types of oligonucleotides which were prepared from formic acid-diphenylamine digests, hydrazinolyzed digests and pancreatic DNase digests, respectively. Oligonucleotides from DNase I digests showed potent inhibitory activity with both SLE sera and rabbit sera. However, the inhibitory activities of purine and pyrimidine oligonucleotides were more potent for SLE sera than for rabbit anti-DNA sera. The determinant size estimated for rabbit sera was in the range of tetra-to heptanucleotide, while in SLE sera it was in the range of di-and trinucleotide.
对系统性红斑狼疮（SLE）患者血清和兔抗DNA血清中DNA抗原决定簇大小的差异进行了研究。通过测量分别由甲酸 - 二苯胺消化物、肼解消化物和胰DNA酶消化物制备的三种不同类型的寡核苷酸对[3H]DNA - 抗体结合的抑制作用，进行了半抗原抑制研究。来自DNA酶I消化物的寡核苷酸对SLE血清和兔血清均显示出强效抑制活性。然而，嘌呤和嘧啶寡核苷酸对SLE血清的抑制活性比对兔抗DNA血清更强。估计兔血清的决定簇大小在四到七核苷酸范围内，而在SLE血清中则在二和三核苷酸范围内。
