Alarcon-Segovia D, Fishbein E, Estrada-Parra S
J Rheumatol. 1975 Jun;2(2):172-7.
The antigenic specificities of antinucleic acid antibodies occurring in systemic lupus erythematosus (SLE), chronic active liver disease, and progressive systemic sclerosis (PSS) have been studied by means of haptenic nucleosides and nucleotides coupled to human serum albumin. SLE sera were also tested with dinucleotides. SLE and chronic active liver disease sera showed marked heterogeneity, producing precipitin lines with nucleosides or nucleotides, or both. The reaction might occur with a nucleoside and not with the corresponding nucleotide, or vice versa. The SLE sera reacted to dinucleotides with marked specificity, being able to recognize base sequences or to react with a dinucleotide despite the absence of a reaction with the individual bases. All sera from patients with PSS showed precipitins with RNA, uridine and UMP. PSS sera which reacted with a nucleoside also reacted with the corresponding nucleotide. Antibodies to DNA were found in a smaller proportion of PSS sera than in sera from SLE or chronic active liver disease. Their presence was confirmed by reactivity with thymidine and TMP.
通过将半抗原核苷和核苷酸与人血清白蛋白偶联，对系统性红斑狼疮（SLE）、慢性活动性肝病和进行性系统性硬化症（PSS）中出现的抗核酸抗体的抗原特异性进行了研究。还用二核苷酸检测了SLE血清。SLE和慢性活动性肝病血清表现出明显的异质性，与核苷或核苷酸或两者都产生沉淀线。反应可能发生在核苷上，而不发生在相应的核苷酸上，反之亦然。SLE血清对二核苷酸有明显的特异性反应，尽管与单个碱基无反应，但仍能识别碱基序列或与二核苷酸反应。所有PSS患者的血清均与RNA、尿苷和UMP产生沉淀素。与核苷反应的PSS血清也与相应的核苷酸反应。与SLE或慢性活动性肝病血清相比，PSS血清中发现抗DNA抗体的比例较小。通过与胸腺嘧啶核苷和TMP的反应证实了它们的存在。
