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胰岛素刺激分离的大鼠肝细胞中可溶性蛋白质的合成。

Insulin stimulates synthesis of soluble proteins in isolated rat hepatocytes.

作者信息

Clark R L, Hansen R J

出版信息

Biochem J. 1980 Sep 15;190(3):615-9. doi: 10.1042/bj1900615.

DOI:10.1042/bj1900615
PMID:7008783
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1162138/
Abstract

The incorporation of [3H]leucine into soluble cellular protein was measured in isolated hepatocytes at extracellular leucine concentrations ranging from 0.15 to 20.0 mM. Insulin caused a 12--15% stimulation of [3H]leucine incorporation in the presence of high extracellular leucine concentrations. It is concluded that insulin causes a small but significant increase in the rate of hepatic protein synthesis.

摘要

在细胞外亮氨酸浓度范围为0.15至20.0 mM的分离肝细胞中，测定了[³H]亮氨酸掺入可溶性细胞蛋白的情况。在细胞外亮氨酸浓度较高时，胰岛素使[³H]亮氨酸掺入增加了12%至15%。得出的结论是，胰岛素使肝脏蛋白质合成速率有小幅但显著的增加。

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Insulin stimulates synthesis of soluble proteins in isolated rat hepatocytes.胰岛素刺激分离的大鼠肝细胞中可溶性蛋白质的合成。
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本文引用的文献

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Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752/1).NCTC克隆929（L株）小鼠细胞亚系在一种简单的化学成分确定培养基（MB 752/1）中的快速增殖。
J Natl Cancer Inst. 1959 May;22(5):1003-17. doi: 10.1093/jnci/22.5.1003.
2
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.
3
Gluconeogenesis in the perfused rat liver.灌注大鼠肝脏中的糖异生作用。
Biochem J. 1966 Nov;101(2):284-92. doi: 10.1042/bj1010284.
4
Inhibition by insulin of valine turnover in liver. Evidence for a general control of proteolysis.胰岛素对肝脏中缬氨酸周转的抑制作用。蛋白质水解普遍调控的证据。
J Biol Chem. 1970 May 10;245(9):2375-83.
5
Compartmentation of free valine and its relation to protein turnover in perfused rat liver.游离缬氨酸的区室化及其与灌注大鼠肝脏蛋白质周转的关系。
J Biol Chem. 1972 May 10;247(9):2776-84.
6
High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study.大鼠肝脏实质细胞分离的高效制备：生化与精细结构研究
J Cell Biol. 1969 Dec;43(3):506-20. doi: 10.1083/jcb.43.3.506.
7
Fatty acid synthetase of developing brain and liver. Content, synthesis, and degradation during development.发育中大脑和肝脏的脂肪酸合成酶。发育过程中的含量、合成及降解
J Biol Chem. 1973 Apr 10;248(7):2502-13.
8
On the mechanism of glucose-6-phosphate dehydrogenase regulation in mouse liver. 3. The rate of enzyme synthesis and degradation.小鼠肝脏中葡萄糖-6-磷酸脱氢酶的调节机制。3. 酶的合成与降解速率
Eur J Biochem. 1974 Jun 1;45(1):211-21. doi: 10.1111/j.1432-1033.1974.tb03545.x.
9
Hormonal modulation of amino acid transport in rat hepatoma cells in tissue culture.组织培养中大鼠肝癌细胞氨基酸转运的激素调节
J Biol Chem. 1973 Feb 25;248(4):1248-54.
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Assessment of protein turnover in perfused rat liver. Evidence for amino acid compartmentation from differential labeling of free and tRNA-gound valine.灌注大鼠肝脏中蛋白质周转的评估。游离缬氨酸和tRNA结合缬氨酸差异标记表明氨基酸存在区室化的证据。
J Biol Chem. 1976 Mar 10;251(5):1375-84.