Rasey J S, Nelson N J
In Vitro. 1980 Jul;16(7):547-53. doi: 10.1007/BF02618377.
EMT-6 tumors were treated in vivo with 300 kVp X-rays, cyclophosphamide, or bleomycin. Tumor cell suspensions were prepared by digesting tumors with trypsin or a collagenase-deoxyribonuclease-pronase cocktail, and cells were plated in vitro for determination of fractional cell survival. Cell survival after X-rays was identical for the two disaggregation methods. Trypsin-derived cells were far more sensitive to bleomycin but less sensitive to cyclophosphamide than those prepared with the mixed enzyme cocktail. Interaction of drug produced and enzyme caused damage was the probable cause for these discrepancies. The nature of the interaction may be drug specific and therefore unpredictable. The results were unlikely to be due to different nonrepresentative tumor cell samples being produced by the two digestion methods, because the X-ray cell survival curves were so similar for the two products.
用300千伏峰值X射线、环磷酰胺或博来霉素对EMT - 6肿瘤进行体内治疗。通过用胰蛋白酶或胶原酶 - 脱氧核糖核酸酶 - 链霉蛋白酶混合物消化肿瘤来制备肿瘤细胞悬液，并将细胞接种到体外以测定细胞分数存活率。两种解离方法对X射线后的细胞存活率相同。与用混合酶混合物制备的细胞相比，胰蛋白酶处理得到的细胞对博来霉素更为敏感，但对环磷酰胺的敏感性较低。药物产生的相互作用和酶引起的损伤可能是这些差异的原因。相互作用的性质可能具有药物特异性，因此无法预测。结果不太可能是由于两种消化方法产生了不同的非代表性肿瘤细胞样本，因为两种产物的X射线细胞存活曲线非常相似。
