Shimbo T, Yata J, Okada H
Int J Cancer. 1978 Oct 15;22(4):422-5. doi: 10.1002/ijc.2910220409.
Non-specific activation of complement (NAC) on cell membranes via the alternative pathway was studied by using leukemic cells and cells from a generalized reticulohistiocytosis. The cells were treated with normal human serum in veronal-buffered saline containing ethyleneglycoltetraacetrate and MG++. Since human erythrocytes (HuE) are known to adhere to complement-reacted cell membranes in an immune adherence reaction, complement activation on the cell membrane was confirmed by the rosette formation of HuE which is due to the generation of C3b molecules on the cell membrane. Only cells from Schilling-type acute monocytic leukemias and cells from a generalized reticulohistiocytosis possessed NAC ability. All other leukemic cells tested, as well as normal hematopoietic and lymphoreticular cells, were NAC-negative. Furthermore, none of the mitogens tested generated NAC ability on normal peripheral blood lymphocytes.
通过替代途径在细胞膜上进行的补体非特异性激活（NAC），使用白血病细胞和全身性网状组织细胞增多症患者的细胞进行了研究。这些细胞在含有乙二醇四乙酸盐和镁离子的巴比妥缓冲盐溶液中用正常人血清处理。由于已知人类红细胞（HuE）在免疫黏附反应中会黏附于补体反应的细胞膜，因此通过HuE的玫瑰花结形成证实了细胞膜上的补体激活，这是由于细胞膜上产生了C3b分子。只有席林型急性单核细胞白血病患者的细胞和全身性网状组织细胞增多症患者的细胞具有NAC能力。所有其他测试的白血病细胞以及正常造血细胞和淋巴网状细胞均为NAC阴性。此外，所测试的有丝分裂原均未在正常外周血淋巴细胞上产生NAC能力。
