Poljak-Blazi M, Slijepcević M, Boranić M
Exp Hematol. 1980 Feb;8(2):174-8.
Experimental diabetes was induced in mice by an intravenous injection of alloxan, 75 mg/kg. Bone marrow cells (8 X 10(4)) from nondiabetic or diabetic mice, 2, 14 or 30 days after induction of diabetes, were injected into lethally irradiated (850 R) nondiabetic or diabetic recipients, and the development of splenic hemopoietic colonies was determined 8 days later. In nondiabetic recipients, bone marrow cells from both types of the donors formed equal numbers of spleen colonies. In diabetic recipients, bone marrow cells from nondiabetic mice produced fewer colonies, whereas bone marrow cels from donors that had diabetes for 14 or 30 days formed as many colonies as in nondiabetic mice. Daily injections of insulin into diabetic recipients improved the colony producing capacity of nondiabetic bone marrow cells. We conclude that bone marrow stem cells (CFUs) acquire resistance to adverse metabolic conditions of hypoinsulinemia and hyperglycemia. Adapted bone marrow cells function well in either the diabetic or nondiabetic environment.
通过静脉注射75毫克/千克的四氧嘧啶诱导小鼠患实验性糖尿病。在糖尿病诱导后2、14或30天，将来自非糖尿病或糖尿病小鼠的骨髓细胞（8×10⁴个）注入接受过致死剂量照射（850拉德）的非糖尿病或糖尿病受体小鼠体内，8天后测定脾造血集落的发育情况。在非糖尿病受体小鼠中，两种供体的骨髓细胞形成的脾集落数量相等。在糖尿病受体小鼠中，来自非糖尿病小鼠的骨髓细胞产生的集落较少，而来自患糖尿病14天或30天的供体的骨髓细胞形成的集落数量与非糖尿病小鼠相同。每天给糖尿病受体小鼠注射胰岛素可提高非糖尿病骨髓细胞的集落生成能力。我们得出结论，骨髓干细胞（CFUs）获得了对低胰岛素血症和高血糖等不良代谢状况的抗性。适应后的骨髓细胞在糖尿病或非糖尿病环境中均能良好发挥功能。
