Cummins C M, Gaber R F, Culbertson M R, Mann R, Fink G R
Genetics. 1980 Aug;95(4):855-79. doi: 10.1093/genetics/95.4.855.
Suppressors of ICR-induced mutations that exhibit behavior similar to bacterial frameshift suppressors have been identified in the yeast Saccharomyces cerevisiae. The yeast suppressors have been divided into two groups. Previous evidence indicated that suppressors of one group (Group II: SUF1, SUF3, SUF4, SUF5 and SUF6) represent mutations in the structural genes for glycyl-tRNA's. Suppressors of the other group (Group III: SUF2 and SUF7) were less well characterized. Although they suppressed some ICR-revertible mutations, they failed to suppress Group II frameshift mutations. This communication provides a more thorough characterization of the Group III suppressors and describes the isolation and properties of four new suppressors in that group (SUF8, SUF9, SUF10 and suf11).--In our original study, Group III suppressors were isolated as revertants of the Group III mutations his4-712 and his4-713. All suppressors obtained as ICR-induced revertants of these mutations mapped at the SUF2 locus near the centromere of chromosome III. Suppressors mapping at other loci were obtained in this study by analyzing spontaneous and UV-induced revertants of the Group III mutations. SUF2 and SUF10 suppress both Group III his4 mutations, whereas SUF7, SUF8, SUF9 and suf11 suppress his4-713, but not his4-712. All of the suppressors except suf11 are dominant in diploids homozygous for his4-713. The suppressors fail to suppress representative UAA, UAG and UGA nonsense mutations.--SUF9 is linked to the centromere of chromosome VI, and SUF10 is linked to the centromere of chromosome XIV. A triploid mapping procedure was used to determine the chromosome locations of SUF7 and SUF8. Subsequent standard crosses revealed linkage of SUF7 to cdc5 on chromosome Xiii and linkage of SUF8 to cdc12 and pet3 on chromosome VIII.
在酿酒酵母中已鉴定出ICR诱导突变的抑制子，其表现出与细菌移码抑制子相似的行为。酵母抑制子已被分为两组。先前的证据表明，一组抑制子（第二组：SUF1、SUF3、SUF4、SUF5和SUF6）代表甘氨酰 - tRNA结构基因中的突变。另一组抑制子（第三组：SUF2和SUF7）的特征不太明确。尽管它们抑制了一些ICR可回复突变，但未能抑制第二组移码突变。本通讯对第三组抑制子进行了更全面的表征，并描述了该组中四个新抑制子（SUF8、SUF9、SUF10和suf11）的分离及特性。——在我们最初的研究中，第三组抑制子是作为第三组突变his4 - 712和his4 - 713的回复子分离得到的。所有作为这些突变的ICR诱导回复子获得的抑制子都定位于第三条染色体着丝粒附近的SUF2位点。在本研究中，通过分析第三组突变的自发和紫外线诱导回复子，获得了定位于其他位点的抑制子。SUF2和SUF10抑制第三组的his4两个突变，而SUF7、SUF8、SUF9和suf11抑制his4 - 713，但不抑制his4 - 712。除suf11外，所有抑制子在his4 - 713纯合二倍体中都是显性的。这些抑制子不能抑制代表性的UAA、UAG和UGA无义突变。——SUF9与第六条染色体的着丝粒相连，SUF10与第十四条染色体的着丝粒相连。采用三倍体定位程序确定了SUF7和SUF8的染色体位置。随后的标准杂交显示SUF7与第十三条染色体上的cdc5连锁，SUF8与第八条染色体上的cdc12和pet3连锁。
