Ono B I, Fujimoto R, Ohno Y, Maeda N, Tsuchiya Y, Usui T, Ishino-Arao Y
Laboratory of Environmental Hygiene Chemistry, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Genetics. 1988 Jan;118(1):41-7. doi: 10.1093/genetics/118.1.41.
Sixty independent UGA suppressors of Saccharomyces cerevisiae have been studied. They are dominant and are divided into 16 groups (loci) by recombination. Suppressors representing these loci are divided into two classes by action spectra; four in class 1 (a broad action spectrum) and 12 in class 2 (a narrow action spectrum). Class 1 suppressors are less frequent in terms of not only total number but also number per locus than class 2 suppressors, indicating difference in either or both mutation frequency and selective pressure between suppressors of the two classes. Two of the class 1 suppressors, SUP152 and SUP161, do not recombine with SUP28 and SUP33, leucine-inserting UAA suppressors, respectively, indicating that they are mutations in genes coding for tRNA(Leu)UUA. Of the remaining two class 1 suppressors, SUP160 which causes lethality in the psi+ cytoplasm is mapped on chromosome XV very close to the centromere, and SUP165 on the right arm of chromosome XIV 44 cM distal to lys9. Of the class 2 suppressors, ten do not recombine with one or another of previously known UGA suppressors. The remaining two class 2 suppressors, SUP154 and SUP155, are mapped on the left and right arms of chromosome VII, respectively.
对酿酒酵母的60个独立UGA抑制子进行了研究。它们是显性的，通过重组被分为16个组（位点）。代表这些位点的抑制子根据作用光谱分为两类；第1类有4个（宽作用光谱），第2类有12个（窄作用光谱）。第1类抑制子不仅在总数上，而且在每个位点的数量上都比第2类抑制子少，这表明两类抑制子在突变频率和选择压力中的一个或两个方面存在差异。第1类抑制子中的两个，SUP152和SUP161，分别不与亮氨酸插入型UAA抑制子SUP28和SUP33重组，这表明它们是编码tRNA(Leu)UUA的基因突变。在其余两个第1类抑制子中，在psi+细胞质中导致致死性的SUP160定位在第十五号染色体上，非常靠近着丝粒，而SUP165在第十四号染色体右臂上，距lys9远端44 cM。在第2类抑制子中，有10个不与先前已知的UGA抑制子中的一个或另一个重组。其余两个第2类抑制子，SUP154和SUP155，分别定位在第七号染色体的左臂和右臂上。
