Murphy T L, Allison R C, Weisman I M, McCaffree D R, Gray B A
J Appl Physiol Respir Environ Exerc Physiol. 1981 Jan;50(1):178-84. doi: 10.1152/jappl.1981.50.1.178.
There is evidence suggesting a role for platelet serotonin (5-HT) in the immediate pulmonary response to endotoxin in the dog (J. Appl. Physiol. 23: 47, 1967). To further define this role, autologous canine platelets were labeled with 5-[14C]HT in vitro and then reinfused. Subsequently Escherichia coli endotoxin (0.55:B-5, Difco), 2.5 mg/kg, was injected. Within 5 min dynamic compliance (CL) fell by more than 50%, and nonelastic resistance (RL) increased by more than 200%. Despite a 95% decrease in platelet count, less than 10% of the platelet 5-HT was released as determined by changes in the radioactivity of platelet-poor plasma (PPP) prepared from both aortic and pulmonary artery blood. As a positive control, injected of bovine collagen produced a similar decrease in platelet count that was associated with a significant increase in the radioactivity of aortic and pulmonary artery PPP. FInally, rapid injection of a dose of 5-HT equivalent to 25% of the 5-HT in circulating platelets did not cause a change in CL or RL equivalent to that produced by endotoxin. From these data we conclude that endotoxin injection does not cause immediate massive platelet activation and that platelet 5-HT does not play a major role in the immediate pulmonary response to endotoxin.
有证据表明，血小板5-羟色胺（5-HT）在犬对内毒素的即时肺部反应中起作用（《应用生理学杂志》23: 47, 1967）。为了进一步明确这一作用，将自体犬血小板在体外用5-[14C]HT标记，然后再输注回去。随后注射大肠杆菌内毒素（0.55:B-5，Difco），剂量为2.5mg/kg。在5分钟内，动态顺应性（CL）下降超过50%，非弹性阻力（RL）增加超过200%。尽管血小板计数下降了95%，但通过对从主动脉和肺动脉血制备的血小板贫浆（PPP）放射性变化的测定，发现释放的血小板5-HT不到10%。作为阳性对照，注射牛胶原蛋白导致血小板计数出现类似下降，同时主动脉和肺动脉PPP的放射性显著增加。最后，快速注射相当于循环血小板中5-HT 25%剂量的5-HT，并未引起与内毒素产生的CL或RL变化相当的改变。根据这些数据，我们得出结论，注射内毒素不会立即导致大量血小板激活，并且血小板5-HT在对内毒素的即时肺部反应中不起主要作用。
