鸡肾制剂对蝶啶的酶促合成。

The enzymatic synthesis of sepiapterin by chicken kidney preparations.

作者信息

Tanaka K, Akino M, Hagi Y, Doi M, Shiota T

出版信息

J Biol Chem. 1981 Mar 25;256(6):2963-72.

Abstract

A chicken kidney preparation which catalyzes the conversion of D-erythro-dihydroneopterin triphosphate to dihydrobiopterin in the presences of Mg2+ and NADPH has been fractionated into three fractions, A2, A1, and B. Fraction A2 in the presence of Mg2+ catalyzes the conversion of D-erythro-dihydroneopterin triphosphate to an unknown intermediate designated compound X. Fraction A1 in the presence of NADPH catalyzes the conversion of compound X to sepiapterin. Fraction B, in the presence of NADPH, catalyzes the conversion of sepiapterin to dihydrobiopterin. Fraction B which is sepiapterin reductase was not studied any further. Fraction A1 is heat-labile and its molecular weight is estimated to be 3.0 x 10(4), whereas fraction A2 is heat-stable and its molecular weight, 7.7 x 10(4). Compound X is labile and its degradation products have been identified to be pterin and pyruvic acid. This information suggests that compound X is 6-(1,2-dioxopropyl)-7,8-dihydropterin.

摘要

一种在Mg2+和NADPH存在下催化D-赤藓型二氢新蝶呤三磷酸转化为二氢生物蝶呤的鸡肾制剂已被分离成三个组分，A2、A1和B。在Mg2+存在下，组分A2催化D-赤藓型二氢新蝶呤三磷酸转化为一种未知中间体，命名为化合物X。在NADPH存在下，组分A1催化化合物X转化为蝶啶。在NADPH存在下，组分B催化蝶啶转化为二氢生物蝶呤。未对作为蝶啶还原酶的组分B进行进一步研究。组分A1对热不稳定，其分子量估计为3.0×10(4)，而组分A2对热稳定，其分子量为7.7×10(4)。化合物X不稳定，其降解产物已被鉴定为蝶呤和丙酮酸。该信息表明化合物X是6-(1,2-二氧代丙基)-7,8-二氢蝶呤。

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