Holmes R L, Harada W A
J Clin Microbiol. 1981 Feb;13(2):279-82. doi: 10.1128/jcm.13.2.279-282.1981.
A rapid technique used for the identification of Streptococcus agalactiae, Lancefield group B, from the blood cultures of two neonatal infants is reported. The method utilized the Phadebact Streptococcus Test System (Pharmacia Diagnostics, Piscataway, N.J.) and the supernatant from 13- and 14-h blood cultures. Additional studies with simulated neonatal blood cultures revealed that this method was reproducible. Additional studies also revealed that some non-specific agglutination did occur, which could not be eliminated with dithiothreitol, but was visibly reduced by treatment with soluble staphylococcal protein A.
本文报道了一种快速技术,用于从两名新生儿的血培养物中鉴定无乳链球菌(兰斯菲尔德B组)。该方法使用了Phadebact链球菌检测系统(法玛西亚诊断公司,新泽西州皮斯卡塔韦)和13至14小时血培养物的上清液。对模拟新生儿血培养物的进一步研究表明,该方法具有可重复性。进一步研究还表明,确实发生了一些非特异性凝集,二硫苏糖醇无法消除这种凝集,但用可溶性葡萄球菌蛋白A处理后,凝集明显减少。