人血浆纤连蛋白的竞争性酶免疫测定法。

Competitive enzyme immunoassay for human plasma fibronectin.

作者信息

Vuento M, Salonen E, Pasanen M, Stenman U H

出版信息

J Immunol Methods. 1981;40(1):101-8. doi: 10.1016/0022-1759(81)90085-5.

Abstract

A competitive enzyme immunoassay for the determination of fibronectin in plasma is described. An enzyme conjugate prepared by coupling alkaline phosphatase to rabbit anti-human fibronectin antibodies by glutaraldehyde was used as principal reagent. The assay was performed by coating polystyrene tubes with purified fibronectin and reacting these coated tubes with a mixture of sample and enzyme-labeled antibodies. After overnight incubation, the amount of enzyme activity associated with the tube was determined. An assay range of 0.5-20 microgram/ml of fibronectin was obtained. The mean concentration of plasma fibronectin in female patients was found to be 270 microgram/ml (standard deviation 50 microgram/ml, n = 22). Denatured fibronectin had low activity in the assay. The presence of cross-reacting antigens in rat and guinea pig plasma was demonstrated by the enzyme immunoassay technique.

摘要

本文描述了一种用于测定血浆中纤连蛋白的竞争性酶免疫测定法。通过戊二醛将碱性磷酸酶与兔抗人纤连蛋白抗体偶联制备的酶结合物用作主要试剂。该测定法是通过用纯化的纤连蛋白包被聚苯乙烯管，并使这些包被管与样品和酶标记抗体的混合物反应来进行的。过夜孵育后，测定与管相关的酶活性量。获得了0.5 - 20微克/毫升纤连蛋白的测定范围。发现女性患者血浆纤连蛋白的平均浓度为270微克/毫升（标准差50微克/毫升，n = 22）。变性纤连蛋白在该测定法中活性较低。酶免疫测定技术证明了大鼠和豚鼠血浆中存在交叉反应抗原。