一种用于检测明胶结合纤连蛋白的竞争性抑制测定法。

A competitive inhibition assay for gelatin binding fibronectin.

作者信息

Doran J E, Callaway B D, Reese A C, Wynn J J, Mansberger A R

出版信息

Vox Sang. 1983;45(3):243-51. doi: 10.1111/j.1423-0410.1983.tb01910.x.

DOI:10.1111/j.1423-0410.1983.tb01910.x

Abstract

A competitive inhibition assay for functional fibronectin (Fn), based on ELISA technology, is described. The assay measures Fn's physiologic ability to bind to denatured collagen (gelatin). Affinity-purified Fn inhibits the binding of alkaline phosphatase coupled Fn to gelatin-coated wells of a microtiter plate in a concentration-dependent manner. The assay range is 50-500 micrograms Fn/ml, which is suitable for the measurement of plasma Fn in both normal and opsonin deficient individuals. It is reproducible over an eightfold dilution of plasma and is resistant to interference by normal plasma proteins. The assay described is quick, quantitative, and reproducible, and satisfies the need for a measure of functional Fn activity in the clinical laboratory.

摘要

本文描述了一种基于酶联免疫吸附测定（ELISA）技术的功能性纤连蛋白（Fn）竞争性抑制测定法。该测定法检测Fn与变性胶原蛋白（明胶）结合的生理能力。亲和纯化的Fn以浓度依赖性方式抑制碱性磷酸酶偶联的Fn与微量滴定板上明胶包被孔的结合。测定范围为50 - 500微克Fn/毫升，适用于正常个体和调理素缺乏个体血浆Fn的测量。该方法在血浆八倍稀释范围内可重复，且不受正常血浆蛋白的干扰。所描述的测定法快速、定量且可重复，满足了临床实验室对功能性Fn活性测量的需求。