Liubavich E M, Naumchuk P L
Ukr Biokhim Zh. 1977 May-Jun;49(3):3-9.
Intensity of skin and blood serum soluble proteins renewal under conditions of vitamin A deficiency in animal organism was studied with application of the label incorporation into the electrophoretic fractions of proteins 1,3 and 24 h after administration of 14C-lysine. Metabolism of collagen proteins, amino acid composition of skin soluble proteins, content of free amino acids and nucleic acid in skin were also examined. With vitamin A deficiency the intensity of 14C-lysine incorporation into the globulin fractions of serum proteins is 2-4 times as high and into the corresponding fractions of skin proteins is 3-5 times as low as in the control. Metabolism of gamma-globulins and collagen proteins of skin under conditions of vitamin A deficiency is found to be slow. The content of DNA and RNA in skin of the avitaminous animals is reliably 17 and 23% lower, respectively. In the skin extracts obtained by means of 0.15 M NaCl the content of free amino acids with vitamin A deficiency increases. No significant changes are found in the amino acid composition of skin proteins soluble in 0.15 M NaCl, with the exception for a 20% decrease in the content of cystine together with cystein.
在动物机体维生素A缺乏的条件下，通过在给予14C-赖氨酸后1、3和24小时将标记物掺入蛋白质的电泳组分，研究了皮肤和血清可溶性蛋白质更新的强度。还检测了皮肤中胶原蛋白的代谢、皮肤可溶性蛋白质的氨基酸组成、游离氨基酸含量和核酸含量。维生素A缺乏时，血清蛋白质球蛋白组分中14C-赖氨酸的掺入强度比对照组高2至4倍，而皮肤蛋白质相应组分中的掺入强度比对照组低3至5倍。发现在维生素A缺乏条件下，皮肤γ-球蛋白和胶原蛋白的代谢缓慢。维生素A缺乏动物皮肤中的DNA和RNA含量分别可靠地降低了17%和23%。在通过0.15M NaCl获得的皮肤提取物中，维生素A缺乏时游离氨基酸的含量增加。在0.15M NaCl中可溶的皮肤蛋白质的氨基酸组成没有发现显著变化，除了胱氨酸和半胱氨酸的含量一起下降了20%。
