Parr E L, Bowen K M, Lafferty K J
Transplantation. 1980 Aug;30(2):135-41. doi: 10.1097/00007890-198008000-00012.
Islets of Langerhans cultured 7 days in vitro no longer contained any capillary endothelial cells, but their endocrine cells remained ultrastructurally normal up to 14 days. Vascular endothelial cells were also lost from cultured thyroid lobes, but more slowly. Thyroid endothelium was readily identified after 7 days of culture, although many cells appeared to be degenerating, and a few degenerating endothelial cells were still present after 14 days in culture. Erythrocytes and leukocytes in the lumina of thyroid vessels were observed to degenerate at about the same rate as the endothelial cells, while those in islet capillary lumina were largely washed out during isolation of the islets. Thyroid lymphatic endothelium and the numerous adipose cells present in this tissue also degenerated during the culture period. Follicle epithelial cells remained viable throughout the culture period, but the number of colloid droplets and endocytic vesicles they contained was markedly, decreased. Thyroid fibroblasts remained viable and appeared to enlarge and accumulate dense granules during culture. These cells were a prominent feature of thyroid lobes after 14 days of culture. Parathyroid tissue associated with the thyroid lobes showed viable endocrine cells but a loss of vascular endothelium after 14 days in culture. The loss of blood leukocytes and vascular endothelial cells in probably the major factor in the altered behavior of thyroid and islet allografts after culture in vitro.
体外培养7天的胰岛不再含有任何毛细血管内皮细胞，但其内分泌细胞在长达14天的时间里超微结构仍保持正常。培养的甲状腺叶也失去了血管内皮细胞，但速度较慢。培养7天后仍可轻易识别出甲状腺内皮，尽管许多细胞似乎正在退化，培养14天后仍有一些退化的内皮细胞存在。观察到甲状腺血管腔中的红细胞和白细胞与内皮细胞以大致相同的速度退化，而胰岛毛细血管腔中的红细胞和白细胞在胰岛分离过程中大部分被冲走。甲状腺淋巴管内皮以及该组织中存在的大量脂肪细胞在培养期间也发生了退化。在整个培养期间，滤泡上皮细胞保持存活，但它们所含的胶体滴和内吞小泡数量明显减少。甲状腺成纤维细胞在培养期间保持存活，并且似乎在培养过程中增大并积累致密颗粒。这些细胞是培养14天后甲状腺叶的一个突出特征。与甲状腺叶相关的甲状旁腺组织在培养14天后显示内分泌细胞存活，但血管内皮丧失。血液白细胞和血管内皮细胞的丧失可能是体外培养后甲状腺和胰岛同种异体移植物行为改变的主要因素。
