Parr E L, Lafferty K J, Bowen K M, McKenzie I F
Transplantation. 1980 Aug;30(2):142-8. doi: 10.1097/00007890-198008000-00013.
Mouse thyroid tissue was dissociated with collagenase, fixed in periodate-lysine-paraformaldehyde (PLP), and further dissociated with EDTA and trypsin to yield cell suspensions containing mainly follicle epithelial cells and vascular endothelial cells. H-2 complex antigens were detected on the vascular endothelial cells at about the same high density as on peritoneal macrophages, and at a lower concentration on the laterobasal membranes of follicle epithelial cells. Neither of these cell types expressed detectable Ia antigens, but a minor cell type was presented that showed dense expression of Ia antigens. This cell type was probably a passenger leukocyte. It showed ultrastructural characteristics closely resembling those of spleen dendritic cells, which are known to express Ia antigens and to be potent stimulator cells in mixed lymphocyte culture. Dissociation of thyroid glands that had been cultured in vitro for 14 days yielded only follicle epithelium, and these cells showed the same labeling density of H-2 complex antigens as on uncultured cells. Dissociation of islets of Langerhans yielded capillary endothelial cells and beta cells, neither of which expressed detectable Ia antigens. The labeling results are discussed in relation to the cellular changes that occur during culture in vitro and the altered behavior of cultured allografts.
将小鼠甲状腺组织用胶原酶解离，固定于高碘酸盐 - 赖氨酸 - 多聚甲醛（PLP）中，再用乙二胺四乙酸（EDTA）和胰蛋白酶进一步解离，以产生主要包含滤泡上皮细胞和血管内皮细胞的细胞悬液。在血管内皮细胞上检测到的H - 2复合体抗原密度与腹膜巨噬细胞上的大致相同，而在滤泡上皮细胞的侧基膜上浓度较低。这两种细胞类型均未表达可检测到的Ia抗原，但发现一种少量的细胞类型显示出Ia抗原的密集表达。这种细胞类型可能是过客白细胞。它显示出的超微结构特征与脾树突状细胞的极为相似，已知脾树突状细胞表达Ia抗原且在混合淋巴细胞培养中是有效的刺激细胞。对体外培养14天的甲状腺进行解离仅产生滤泡上皮，并且这些细胞显示出与未培养细胞相同的H - 2复合体抗原标记密度。对胰岛进行解离产生毛细血管内皮细胞和β细胞，两者均未表达可检测到的Ia抗原。结合体外培养过程中发生的细胞变化以及培养的同种异体移植物行为的改变对标记结果进行了讨论。
