通过大肠杆菌转化试验证明质粒DNA被完整植物（小浮萍）摄取的证据。
Evidence for uptake of plamid DNA into intact plants (Lemna perpusilla) proved by an E. coli transformation assay.
作者信息
Frey M, Rall S, Roth A, Hemleben V
出版信息
Z Naturforsch C Biosci. 1980 Nov-Dec;35(11-12):1104-6. doi: 10.1515/znc-1980-11-1243.
PMID:7010814
Abstract
The water plant Lemna perpusilla has been incubated with the E. coli plasmids pMB9 and pBR325, respectively. Uptake of plasmids has been shown by subsequent transformation of E. coli cells to tetracycline resistance after treatment with Lemna DNA from plasmid-incubated plants. In 7 out of 15 assays we found stable transformants. From the transformation rate an amount of 10(-4) to 10(-6) micrograms plasmid DNA per 10 micrograms of plant DNA can be calculated.
摘要
水生植物小浮萍已分别与大肠杆菌质粒pMB9和pBR325一起进行培养。在用来自经质粒培养的植物的浮萍DNA处理后，大肠杆菌细胞随后被转化为对四环素具有抗性，这表明了质粒的摄取。在15次试验中的7次中，我们发现了稳定的转化体。根据转化率，可以计算出每10微克植物DNA中有10^(-4)到10^(-6)微克的质粒DNA。
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