Heisler N, Weitz A M
Respir Physiol. 1976 Apr;26(2):249-63. doi: 10.1016/0034-5687(76)90103-1.
Larger Spotted Dogfish, Scyliorhinus stellaris, were exposed to varied ambient temperature (t) in order to determine the behavior of extracellular pH (pHe), Pco2 and bicarbonate concentration as well as intracellular pH (pHi) in three muscle types. pHe was found to vary with temperature slightly less than expected on the basis of the rule of constant relative alkalinity in juvenile (deltapH/deltat= -0.0148 per degree centigrade) as well as in adult (deltapH/deltat= -0.0136) fish. The absolute pHe values of adult fish were about 0.08 pH units higher than in juvenile fish. Arterial pco2 increased with rising temperature, the increase being much more marked in adult than in juvenile fish. Extracellular bicarbonate concentration (calculated from the pH and Pco2 values measured in arterial blood) was not maintained constant, but diminished in juvenile and increased in adult fish with increasing temperature, indicating that extracellular pH in dogfish is regulated by variations of both Pco2 and bicarbonate concentration. Variations of intracellular pH with temperature (deltapHi/deltat), -0.0178 for white muscle, -0.0334 for red muscle, and -0.0098 for heart muscle, were significantly different from the values of the extracellular compartment and, except for white muscle, significantly different from the condition for constant relative alkalinity (deltapH/deltat= -0.0183). These results are in agreement with the rule of constant relative alkalinity with respect to extracellular pH and possibly also with respect to an overall mean intracellular pH, but the rule is not quantitatively followed by the individual body compartments and tissues.
为了确定三种肌肉类型中细胞外pH值（pHe）、二氧化碳分压（Pco2）、碳酸氢盐浓度以及细胞内pH值（pHi）的变化情况，将大斑星鲨（Scyliorhinus stellaris）暴露于不同的环境温度（t）下。研究发现，在幼鱼（ΔpH/Δt = -0.0148/℃）和成鱼（ΔpH/Δt = -0.0136）中，pHe随温度的变化略小于基于恒定相对碱度规则的预期值。成鱼的绝对pHe值比幼鱼高约0.08个pH单位。动脉血二氧化碳分压随温度升高而增加，成鱼的增加幅度比幼鱼更为明显。细胞外碳酸氢盐浓度（根据动脉血中测得的pH值和二氧化碳分压值计算得出）并非保持恒定，而是在幼鱼中随温度升高而降低，在成鱼中则随温度升高而增加，这表明星鲨的细胞外pH值受二氧化碳分压和碳酸氢盐浓度变化的共同调节。细胞内pH值随温度的变化（ΔpHi/Δt），白肌为-0.0178，红肌为-0.0334，心肌为-0.0098，与细胞外部分的值显著不同，除白肌外，也与恒定相对碱度条件下的值（ΔpH/Δt = -0.0183）显著不同。这些结果在细胞外pH值方面符合恒定相对碱度规则，在整体平均细胞内pH值方面可能也符合该规则，但各个身体部分和组织并未在数量上严格遵循该规则。
