Hagberg L, Jodal U, Korhonen T K, Lidin-Janson G, Lindberg U, Svanborg Edén C
Infect Immun. 1981 Feb;31(2):564-70. doi: 10.1128/iai.31.2.564-570.1981.
The capacity of 453 Escherichia coli strains to agglutinate erythrocytes and yeast cells and to attach to human urinary tract epithelial cells was tested. The strains were isolated from the urine of patients with acute pyelonephritis, acute cystitis, or asymptomatic bacteriuria and from the stools of healthy school children. Three main patterns of hemagglutination were found: (i) mannose-resistant agglutination of human erythrocytes alone or simultaneously with mannose-sensitive agglutination of guinea pig erythrocytes; (ii) only mannose-sensitive agglutination of guinea pig and other erythrocytes; and (iii) no agglutination. Strains with mannose-resistant agglutination of human erythrocytes alone or in combination with mannose-sensitive hemagglutination attached in high numbers to human urinary tract epithelial cells. Bacteria inducing only mannose-sensitive hemagglutination attached in low numbers, and non-agglutinating strains did not bind to the urinary tract epithelial cells. The bacterial surface antigen(s) mediating mannose-resistant hemagglutination of human erythrocytes and attachment to human urinary tract epithelial cells may be one factor selecting for E. coli from among the fecal flora which infect the urinary tract. The highest proportion of strains with this property was found among acute pyelonephritis isolates (77%), and the lowest proportion of strains with this property was found among normal fecal E. coli (16%).
检测了453株大肠杆菌凝集红细胞和酵母细胞以及黏附人尿道上皮细胞的能力。这些菌株分别从急性肾盂肾炎、急性膀胱炎或无症状菌尿患者的尿液以及健康学童的粪便中分离得到。发现了三种主要的血凝模式：（i）仅对人红细胞的甘露糖抗性凝集或同时伴有对豚鼠红细胞的甘露糖敏感性凝集；（ii）仅对豚鼠和其他红细胞的甘露糖敏感性凝集；（iii）无凝集。仅对人红细胞有甘露糖抗性凝集或伴有甘露糖敏感性血凝的菌株大量黏附于人尿道上皮细胞。仅诱导甘露糖敏感性血凝的细菌少量黏附，而不凝集的菌株不与尿道上皮细胞结合。介导人红细胞甘露糖抗性血凝和黏附人尿道上皮细胞的细菌表面抗原可能是从感染尿道的粪便菌群中选择大肠杆菌的一个因素。在急性肾盂肾炎分离株中发现具有该特性的菌株比例最高（77%），而在正常粪便大肠杆菌中发现具有该特性的菌株比例最低（16%）。
