酿酒酵母中的复制后修复
Postreplication repair in Saccharomyces cerevisiae.
作者信息
Resnick M A, Boyce J, Cox B
出版信息
J Bacteriol. 1981 Apr;146(1):285-90. doi: 10.1128/jb.146.1.285-290.1981.
Abstract
Postreplication events in logarithmically growing excision-defective mutants of Saccharomyces cerevisiae were examined after low doses of ultraviolet light (2 to 4 J/m2). Pulse-labeled deoxyribonucleic acid had interruptions, and when the cells were "chased," the interruptions were no longer detected. Since the loss of interruptions was not associated with an exchange of pyrimidine dimers at a detection level of 10 to 20% of the induced dimers, we concluded that postreplication repair in excision-defective mutants (or leaky mutants) does not involve molecular recombination. Pyrimidine dimers were assayed by utilizing the ultraviolet-endonuclease activity in extracts of Micrococcus luteus and newly developed alkaline sucrose gradient techniques, which yielded chromosomal-size deoxyribonucleic acid after treatment of irradiated cells.
摘要
在用低剂量紫外线（2至4焦耳/平方米）照射后，对处于对数生长期的酿酒酵母切除缺陷型突变体的复制后事件进行了研究。脉冲标记的脱氧核糖核酸出现了中断，当细胞进行“追踪”时，中断不再被检测到。由于在诱导二聚体的10%至20%的检测水平下，中断的消失与嘧啶二聚体的交换无关，我们得出结论，切除缺陷型突变体（或渗漏突变体）中的复制后修复不涉及分子重组。利用藤黄微球菌提取物中的紫外线内切酶活性和新开发的碱性蔗糖梯度技术对嘧啶二聚体进行了测定，该技术在处理受辐照细胞后产生了染色体大小的脱氧核糖核酸。
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