酿酒酵母的RAD1和RAD52基因控制着不同类型的重组事件。
Different types of recombination events are controlled by the RAD1 and RAD52 genes of Saccharomyces cerevisiae.
作者信息
Klein H L
机构信息
Department of Biochemistry, New York University, New York 10016.
出版信息
Genetics. 1988 Oct;120(2):367-77. doi: 10.1093/genetics/120.2.367.
Abstract
Intrachromosomal recombination within heteroallelic duplications located on chromosomes III and XV of Saccharomyces cerevisiae has been examined. Both possible orientations of alleles have been used in each duplication. Three recombinant classes, gene conversions, pop-outs and triplications, were recovered. Some of the recombinant classes were not anticipated from the particular allele orientation of the duplication. Recovery of these unexpected recombinants requires the RAD1 gene. These studies show that RAD1 has a role in recombination between repeated sequences, and that the recombination event is a gene conversion associated with a crossover. These events appear to involve very localized conversion of a heteroduplex region and are distinct from RAD52 mediated gene conversion events. Evidence is also presented to suggest that most recombination events between direct repeats are intrachromatid, not between sister chromatids.
摘要
对酿酒酵母染色体III和XV上异等位基因重复序列内的染色体内重组进行了研究。在每个重复序列中都使用了等位基因的两种可能方向。回收了三种重组类型，即基因转换、弹出和三倍体。某些重组类型并非来自重复序列特定的等位基因方向。回收这些意外的重组体需要RAD1基因。这些研究表明，RAD1在重复序列之间的重组中起作用，并且重组事件是与交叉相关的基因转换。这些事件似乎涉及异源双链区域的非常局部的转换，并且与RAD52介导的基因转换事件不同。还提供了证据表明，直接重复序列之间的大多数重组事件发生在染色单体内，而非姐妹染色单体之间。
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