Straus D C, Mattingly S J, Milligan T W, Doran T I, Nealon T J
J Clin Microbiol. 1980 Sep;12(3):421-3. doi: 10.1128/jcm.12.3.421-423.1980.
Six strains of serotype III group B streptococci isolated from confirmed cases of neonatal disease were examined for their ability to produce proteolytic enzymes. Three neuraminidase-producing strains and three non-neuraminidase-producing strains were employed in this study. Protease production was examined in 1,000-fold concentrated filtrates of stationary-phase cells with an insoluble substrate derived from horse hide powder labeled covalently with Remazol brilliant blue. Protease activity was not detected in any cultural supernatant fluids until they were fractionated on Sephadex G-100. After fractionation, the neuraminidase-producing strains were shown to elaborate approximately sixfold more protease than the non-neuraminidase-producing strains. The finding that clinical isolates of group B streptococci that elaborated high levels of neuraminidase also produced elevated levels of extracellular protease may indicate that the production of several different factors may determine the virulence of these organisms.
对从确诊的新生儿疾病病例中分离出的6株B族血清型III链球菌进行了产蛋白水解酶能力的检测。本研究采用了3株产神经氨酸酶菌株和3株不产神经氨酸酶菌株。用共价标记了雷马素亮蓝的马皮粉衍生的不溶性底物，对稳定期细胞1000倍浓缩滤液中的蛋白酶产生情况进行了检测。在任何培养上清液在Sephadex G - 100上进行分级分离之前，均未检测到蛋白酶活性。分级分离后，产神经氨酸酶的菌株所产生的蛋白酶比不产神经氨酸酶的菌株多约6倍。高水平产神经氨酸酶的B族链球菌临床分离株也产生高水平细胞外蛋白酶这一发现，可能表明几种不同因子的产生可能决定了这些生物体的毒力。
