Mochizuki S, Neely J R
J Physiol (Paris). 1980;76(7):805-12.
Insulin treatment of hearts during aerobic reperfusion following transient ischemia in the working rat heart preparation significantly improved the recovery of myocardial function. This improvement was reflected both by a shorter time required for the heart to resume beating, and to increase heart rate and peak systolic pressure after resumption of beating. The beneficial effects of insulin may be related to improved energy metabolism secondary to small increases in pyruvate production during the early phase of reperfusion. It was also associated with an increased rate of restoration of cellular K+. Pyruvate addition to the perfusate also improved resumption of spontaneous beating of the heart and restoration of normal rate and pressure development. This effect of pyruvate was also associated with increased cellular levels of K+. Both insulin and pyruvate may improve ATP production during the first few minutes of reperfusion when glycolysis and oxidation of fatty acids are inhibited, but ATP levels were not increased after 30 min of reperfusion.
在工作大鼠心脏制备模型中，短暂缺血后进行有氧再灌注时，胰岛素治疗心脏可显著改善心肌功能的恢复。这种改善表现为心脏恢复跳动所需时间缩短，以及恢复跳动后心率和收缩压峰值增加。胰岛素的有益作用可能与再灌注早期丙酮酸生成略有增加继发的能量代谢改善有关。它还与细胞钾离子恢复速率增加有关。向灌注液中添加丙酮酸也可改善心脏自发跳动的恢复以及正常心率和压力的恢复。丙酮酸的这种作用也与细胞内钾离子水平增加有关。在糖酵解和脂肪酸氧化受到抑制的再灌注最初几分钟内，胰岛素和丙酮酸均可改善ATP生成，但再灌注30分钟后ATP水平并未升高。
