Dolara P, Barale R, Mazzoli S, Benetti D
Mutat Res. 1980 Nov;79(3):213-21. doi: 10.1016/0165-1218(80)90068-3.
The activation in vitro of the mutagens isolated from beef extract, when tested with Salmonella typhimurium strain TA1538, requires the presence of S9 fractions from livers of PCB- or 3-methylcholanthrene-induced rats. S9 fractions from uninduced rats were unable to activate the mutagens, but no induction was necessary with Swiss albino mice. CD-1 mice had intermediate activation capabilities, which increased after the addition of 0.75% BHA to their diet. Human S9 were poor activators. No activation was observed with the whole homogenates. The active metabolites formed were unstable at 37 degrees C and firmly bound to protein. When isolated livers were perfused for activation, no inherently active mutagens were released into the perfusate. Similarly, no active mutagens were found in the urine of mice after administration to them of beef extract mutagens p.o. or i.p. Intrasanguine host-mediated assay did not show any significant mutagenic effect. The possibility that genotoxic effects of these compounds might be confined to metabolically competent cells of induced animals is discussed.
用鼠伤寒沙门氏菌TA1538菌株检测时，从牛肉浸膏中分离出的诱变剂在体外的激活需要来自多氯联苯或3-甲基胆蒽诱导的大鼠肝脏的S9组分。未诱导大鼠的S9组分无法激活诱变剂，但对于瑞士白化小鼠则无需诱导。CD-1小鼠具有中等激活能力，在其饮食中添加0.75%丁基羟基茴香醚后激活能力增强。人S9是较差的激活剂。用全匀浆未观察到激活现象。形成的活性代谢物在37℃不稳定且与蛋白质紧密结合。当灌注分离的肝脏以进行激活时，没有固有活性的诱变剂释放到灌注液中。同样，给小鼠口服或腹腔注射牛肉浸膏诱变剂后，在其尿液中未发现活性诱变剂。血内宿主介导试验未显示任何显著的诱变作用。讨论了这些化合物的遗传毒性作用可能局限于诱导动物的代谢活性细胞的可能性。
