用于免疫组织化学的抗胆碱乙酰转移酶片段抗原结合（Fab）
Anti-choline acetyltransferase fragments antigen binding (Fab) for immunohistochemistry.
作者信息
Peng J H, Kimura H, McGeer P L, McGeer E G
出版信息
Neurosci Lett. 1981 Feb 6;21(3):281-5. doi: 10.1016/0304-3940(81)90217-2.
PMID:7012696
Abstract
The preparation of fragments antigen binding (Fab) from serum of rabbits immunized against human brain choline acetyltransferase (CAT) is described. Ant-CAT Fab fragments were less potent than anti-CAT immunoglobulin (IgG) in inhibiting mammalian CAT activity and did not precipitate CAT activity. They proved, however, far superior to anti-CAT IgG for the immunohistochemical staining of CAT in mammalian neurons.
摘要
本文描述了从免疫兔血清中制备抗人脑胆碱乙酰转移酶（CAT）的抗原结合片段（Fab）的方法。抗CAT Fab片段在抑制哺乳动物CAT活性方面比抗CAT免疫球蛋白（IgG）效力更低，且不能沉淀CAT活性。然而，在对哺乳动物神经元中CAT进行免疫组织化学染色时，它们被证明远比抗CAT IgG优越。
Zotero 插件