通过在厌氧培养基中添加明胶提高梅毒螺旋体（Nichols株）在体外的运动性和毒力保留。

Enhanced retention of motility and virulence of Treponema pallidum (Nichols strain) in vitro by the addition of gelatin to anaerobic medium.

作者信息

Prpic J K, Trewartha F, Graves S R

出版信息

Sex Transm Dis. 1981 Jan-Mar;8(1):1-4. doi: 10.1097/00007435-198101000-00001.

DOI:10.1097/00007435-198101000-00001

Abstract

The effect of gelatin on the survival of Treponema pallidum (Nichols strain) in anaerobic maintenance medium was investigated by monitoring of the retention of treponemal motility and virulence. Motility of T. pallidum was determined by dark-field microscopy, and virulence of T. pallidum was measured by observation of the latent periods of infection after inoculation of rabbits with virulent organisms. Gelatin concentrations of zero, 3%, 5%, and 15% were tested, and optimal retention of treponemal motility and virulence was observed at 5% and 10% gelatin.

摘要

通过监测梅毒螺旋体运动性和毒力的保持情况，研究了明胶对厌氧维持培养基中梅毒螺旋体（Nichols株）存活的影响。梅毒螺旋体的运动性通过暗视野显微镜测定，梅毒螺旋体的毒力通过用有毒力的生物体接种兔子后观察感染潜伏期来测量。测试了明胶浓度为零、3%、5%和15%的情况，在明胶浓度为5%和10%时观察到梅毒螺旋体运动性和毒力的最佳保持。