Fromtling R A, Fromtling A M, Staib F
Zentralbl Bakteriol A. 1980 Nov;248(2):268-73.
A modified blood residual nitrogen plate auxanographic method was applied to the detection of experimental uremia in a murine model. The yeast-like fungus, Cryptococcus neoformans, was used as the indicator. Transient uremia was induced by injection of 0.2 ml glycerol intramuscularly. The low molecular weight nitrogen levels were estimated by measuring the diameter of the auxanogram at intervals of 2 hr for 24 hr and at 32 hr after the glycerol injection. After 4 hr, elevated levels of low molecular weight nitrogen were found. Maximum levels occurred 20 hr post glycerol injection. This method requires only 5 microliter of whole blood per assay. The results can be read after an incubation time of 24 hr at 26 degree C. The stability of the prepared plates was determined to be at least 96 hr at 4 degree C. The ease of use, reliability and versatility of the modified auxanographic method are discussed.
一种改良的血残余氮平板生长谱法被应用于小鼠模型实验性尿毒症的检测。以酵母样真菌新型隐球菌作为指示菌。通过肌肉注射0.2 ml甘油诱导短暂性尿毒症。在甘油注射后，每隔2小时测量生长谱的直径持续24小时，并在32小时时测量，以此来估算低分子量氮水平。4小时后，发现低分子量氮水平升高。最大水平出现在甘油注射后20小时。该方法每次检测仅需5微升全血。在26℃孵育24小时后即可读取结果。所制备平板在4℃的稳定性被确定为至少96小时。文中讨论了改良生长谱法的易用性、可靠性和多功能性。
