尿毒症对正常及新型隐球菌感染小鼠脾脏细胞淋巴细胞转化和化学发光的影响。
Effect of uremia on lymphocyte transformation and chemiluminescence by spleen cells of normal and Cryptococcus neoformans-infected mice.
作者信息
Fromtling R A, Fromtling A M, Staib F, Müller S
出版信息
Infect Immun. 1981 Jun;32(3):1073-8. doi: 10.1128/iai.32.3.1073-1078.1981.
The effect of uremia on immune incompetence was studied. BALB/c mice were infected with a minimally virulent strain of Cryptococcus neoformans 6 weeks before immune assay. Uremia was induced by intramuscular injection of 0.15 ml of glycerol. Pooled spleen cells from four experimental groups (normal, uremic, infected, and infected and uremic) were assayed by lymphocyte transformation (LT) and luminol-dependent chemiluminescence (CL) 24 h after induction of uremia. A greater response to phytohemagglutinin and concanavalin A stimulation in tests of LT and CL was exhibited by uremic cells than by nonuremic cells; however, the presence of BALB/c uremic serum resulted in lower responses by both LT and CL. Infected mice showed a greater response to mitogens than did noninfected mice, but no significant stimulation in response to heat-killed whole cells of C. neoformans. Spleen cell populations of uremic mice had a lower viability and a different composition of spleen cell subpopulations than did cell preparations from nonuremic mice.
研究了尿毒症对免疫功能不全的影响。在免疫测定前6周，将BALB/c小鼠感染极低毒力的新型隐球菌菌株。通过肌肉注射0.15 ml甘油诱导尿毒症。在诱导尿毒症24小时后，通过淋巴细胞转化（LT）和鲁米诺依赖性化学发光（CL）对四个实验组（正常、尿毒症、感染以及感染合并尿毒症）的脾细胞进行检测。与非尿毒症细胞相比，尿毒症细胞在LT和CL试验中对植物血凝素和刀豆球蛋白A刺激的反应更强；然而，BALB/c尿毒症血清的存在导致LT和CL反应均降低。感染小鼠对丝裂原的反应比未感染小鼠更强，但对热灭活的新型隐球菌全细胞无明显刺激反应。与非尿毒症小鼠的细胞制剂相比，尿毒症小鼠的脾细胞群体活力较低，脾细胞亚群组成不同。
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