Staib F
Zentralbl Bakteriol A. 1981 Feb;248(4):575-8.
To enable studies of the dependence of Cryptococcus neoformans and its perfect and imperfect states upon bird manure as a habitat of this pathogen, a nutrient medium closely resembling natural conditions was prepared. As sole nutrient, the water soluble ingredients of manure from pigeons (Columbia livia) were used. There was no heat sterilization of the manure filtrate. Using a standard pair of C. neoformans strains for mating, it could be demonstrated that the perfect state of the fungus developed on this so called pigeon manure filtrate agar within 48 h at 26 degrees C. This medium is supposed to help in the elucidation of the epidemiological significance of the perfect and imperfect states of this pathogen.
为了能够研究新型隐球菌及其有性态和无性态对鸽粪作为该病原体栖息地的依赖性，制备了一种与自然条件非常相似的营养培养基。作为唯一的营养物质，使用了来自家鸽（Columbia livia）粪便的水溶性成分。粪便滤液未进行热灭菌。使用一对标准的新型隐球菌菌株进行交配，可以证明该真菌的有性态在这种所谓的鸽粪滤液琼脂上于26℃下48小时内发育。该培养基被认为有助于阐明该病原体有性态和无性态的流行病学意义。
