Jones C E, Pike J F, Dickinson R P, Rousseau R J
Am J Clin Pathol. 1981 Apr;75(4):509-18. doi: 10.1093/ajcp/75.4.509.
Fluorescent enzyme immunoassays have been developed for antibody to either single-stranded (ssDNA) or double-stranded (dsDNA) DNA. Preliminary testing of several clinically distinct groups of persons is consistent with the suggestion that the former assay (anti-ssDNA antibody) has sufficient sensitivity and specificity to be used as a generalized test for the detection of possible systemic lupus erythematosus in a heterogeneous clinical population. Further evaluation may ultimately establish its utility in the diagnosis of other related disorders. The sensitivity and specificity of the anti-dsDNA antibody fluorescent enzyme immunoassay are compatible with its use as a confirmatory test in the laboratory diagnosis of systemic lupus erythematosus. Qualitative comparisons between both assays and the more classic laboratory procedures involved in the diagnosis and treatment of systemic lupus erythematosus were quite favorable. The enzyme immunoassay method appears to be acceptably reproducible, and rheumatoid factor has neither a suppressive nor an enhancing effect on test values.
已开发出用于检测单链（ssDNA）或双链（dsDNA）DNA抗体的荧光酶免疫测定法。对几组临床上不同的人群进行的初步测试表明，前一种测定法（抗单链DNA抗体）具有足够的敏感性和特异性，可作为检测异质性临床人群中可能存在的系统性红斑狼疮的通用测试。进一步评估最终可能确定其在诊断其他相关疾病中的效用。抗双链DNA抗体荧光酶免疫测定法的敏感性和特异性使其适用于系统性红斑狼疮实验室诊断的确诊试验。这两种测定法与系统性红斑狼疮诊断和治疗中涉及的更经典实验室程序之间的定性比较结果相当理想。酶免疫测定法似乎具有可接受的重现性，类风湿因子对测试值既无抑制作用也无增强作用。
