Pollard K M, Steele R, Hogg S, Webb J
Rheumatol Int. 1986;6(3):139-44. doi: 10.1007/BF00270351.
Antibodies to double-stranded DNA (dsDNA), generally regarded as highly specific for systemic lupus erythematosus (SLE), have also been reported in chronic active hepatitis (CAH). Using the Farr assay and E. coli DNA, fractionated by benzoylated-naphthoylated-DEAE cellulose chromatography into dsDNA and dsDNA containing single-stranded regions, we compared the serum DNA binding of CAH and SLE patients. Although CAH sera were found to have dsDNA binding significantly above the normal control group such binding was of low level and we could find no evidence of markedly elevated dsDNA binding in CAH. However 12 of the 20 CAH sera studied did bind preferentially to dsDNA containing single-stranded regions suggesting the presence of anti-single-stranded DNA antibodies. We conclude that the description of elevated anti-dsDNA antibodies, as measured by the Farr assay, in CAH is due to the interaction of anti-single-stranded DNA antibodies or other serum components with single-stranded DNA contaminating dsDNA preparations.
通常认为，双链DNA（dsDNA）抗体对系统性红斑狼疮（SLE）具有高度特异性，但慢性活动性肝炎（CAH）患者中也有此类抗体的报道。我们使用Farr检测法，并将通过苯甲酰化-萘甲酰化-二乙氨基乙基纤维素色谱法分离为dsDNA和含有单链区域的dsDNA的大肠杆菌DNA，来比较CAH患者和SLE患者血清与DNA的结合情况。尽管发现CAH患者血清与dsDNA的结合显著高于正常对照组，但这种结合水平较低，且我们未发现CAH患者中dsDNA结合明显升高的证据。然而，在研究的20份CAH患者血清中，有12份确实优先与含有单链区域的dsDNA结合，这表明存在抗单链DNA抗体。我们得出结论，通过Farr检测法测得的CAH患者抗dsDNA抗体升高，是由于抗单链DNA抗体或其他血清成分与污染dsDNA制剂的单链DNA相互作用所致。
