用于临床目的的抗双链DNA和抗单链DNA抗体同步检测评估。
Evaluation of the simultaneous estimation of anti-dsDNA and anti-ssDNA antibodies for clinical purposes.
作者信息
Lange A
出版信息
Clin Exp Immunol. 1978 Mar;31(3):472-81.
The double-antibody solid-phase assay for DNA antibodies permits simultaneous and quantitative determination of antibodies to dsDNA and ssDNA. Using this method, 170 sera, mainly ANA-positive, were examined for the presence of anti-dsDNA and anti-ssDNA to assess the role of these antibodies in the ANA reaction. It was found that in the SLE group of patients, their ability to respond to dsDNA was correlated with the multiorgan symptomatology of disease. Anti-ssDNA titres are also highest in this group. However, anti-ssDNA titres predominate over anti-dsDNA in other collagen diseases. This predominance increases as we progress from the SLE group to undefined mild collagenosis, because the response to dsDNA decreases more than the response to ssDNA. This observation suggests that the clinical manifestation of the collagen diseases and multiorgan manifestation of SLE is linked with the pattern of response to DNA in the majority of cases. In conclusion, it appears that the determination of both ssDNA and dsDNA antibodies can be of value for the prognosis and management of patients with connective tissue disease.
用于检测DNA抗体的双抗体固相分析法可同时对双链DNA（dsDNA）抗体和单链DNA（ssDNA）抗体进行定量测定。采用该方法，对170份主要为抗核抗体（ANA）阳性的血清样本进行检测，以确定抗dsDNA和抗ssDNA的存在情况，从而评估这些抗体在ANA反应中的作用。研究发现，在系统性红斑狼疮（SLE）患者组中，其对dsDNA的反应能力与疾病的多器官症状相关。该组患者的抗ssDNA滴度也最高。然而，在其他胶原病中，抗ssDNA滴度高于抗dsDNA。随着病情从SLE组发展到未明确的轻度胶原病，这种优势愈发明显，因为对dsDNA的反应比对ssDNA的反应下降得更多。这一观察结果表明，在大多数情况下，胶原病的临床表现以及SLE的多器官表现与对DNA的反应模式有关。总之，检测ssDNA和dsDNA抗体似乎对结缔组织病患者的预后评估和治疗具有重要价值。
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