Hussong D, Damaré J M, Weiner R M, Colwell R R
Appl Environ Microbiol. 1981 Jan;41(1):35-45. doi: 10.1128/aem.41.1.35-45.1981.
Aerobic and facultatively anaerobic bacteria isolated from false-positive, presumptive, total coliform, most-probable-number tests of Chesapeake Bay oyster, water, and sediment samples were characterized and then classified by numerical taxonomy. A total of 538 bacterial strains clustered into 17 phena, the predominant groups of which were Enterobacteriaceae (including Escherichia coli), Aeromonas spp., and Bacillus spp. Bacillus spp. were recovered most frequently from sediment samples. Gas-producing strains which were not members of the Enterobacteriaceae were not isolated during this study. However, disproportionately large numbers of atypical and anaerogenic lactose-fermenting strains were encountered. We concluded that no single, specific bacterial group can be identified as being responsible for the false-positive reaction in the presumptive coliform test. Instead, the false-positive reaction is a result of complex interactions among various genera, representing predominantly bacteria other than coliforms.
从切萨皮克湾牡蛎、水和沉积物样本的假阳性、推测性、总大肠菌群、最可能数测试中分离出的需氧和兼性厌氧细菌进行了表征，然后通过数值分类法进行分类。总共538株细菌菌株聚为17个表观群，其中主要的类群是肠杆菌科（包括大肠杆菌）、气单胞菌属和气芽孢杆菌属。芽孢杆菌属在沉积物样本中最常被分离出来。在本研究中未分离出不属于肠杆菌科的产气菌株。然而，遇到了数量不成比例的大量非典型和不产气乳糖发酵菌株。我们得出结论，没有单一的特定细菌类群可被确定为导致推测性大肠菌群测试假阳性反应的原因。相反，假阳性反应是各种属之间复杂相互作用的结果，主要代表非大肠菌群的细菌。
