Palmer C J, Tsai Y L, Lang A L, Sangermano L R
County Sanitation Districts of Orange County, Fountain Valley, California 92728-8127.
Appl Environ Microbiol. 1993 Mar;59(3):786-90. doi: 10.1128/aem.59.3.786-790.1993.
A test that allows for early detection of fecally contaminated coastal water would enhance public health protection. Colilert-Marine Water (Colilert-MW; Environetics, Branford, Conn.) is a rapid 24-h test that has recently been developed to detect total coliforms and Escherichia coli in coastal water. We performed a premarketing evaluation of the Colilert-MW product, testing it in parallel with the multiple tube fermentation (MTF) method for 86 coastal water samples in southern California. Statistical analysis was performed by using paired t tests and linear regression. Bacterial isolates were evaluated by biochemical and genetic analysis. The results of this study showed a strong correlation between the traditional MTF and the Colilert-MW method for detection of total coliforms (r = 0.95) and E. coli (r = 0.89) in ocean water samples. Paired t-test results indicated that the Colilert-MW and MTF were equivalent in detecting E. coli and that the Colilert-MW may be more sensitive in the detection of total coliforms. We conclude that Colilert-MW would be a useful tool with which to monitor coastal beach water.
一种能够早期检测受粪便污染的沿海水域的检测方法将加强对公众健康的保护。Colilert-海水检测试剂盒（Colilert-MW；美国康涅狄格州布兰福德市的Environetics公司）是一种新开发的快速24小时检测方法，用于检测沿海水域中的总大肠菌群和大肠杆菌。我们对Colilert-MW产品进行了上市前评估，将其与多管发酵（MTF）方法同时用于检测南加州86份沿海水样。采用配对t检验和线性回归进行统计分析。通过生化和基因分析对细菌分离株进行评估。本研究结果表明，在海水样本中，传统的MTF方法与Colilert-MW方法在检测总大肠菌群（r = 0.95）和大肠杆菌（r = 0.89）方面具有很强的相关性。配对t检验结果表明，Colilert-MW和MTF在检测大肠杆菌方面等效，而Colilert-MW在检测总大肠菌群方面可能更敏感。我们得出结论，Colilert-MW将是监测沿海滩涂水域的一种有用工具。
