大肠杆菌在适应低水平镉离子过程中的镉结合成分。
Cadmium-binding component in Escherichia coli during accommodation to low levels of this ion.
作者信息
Khazaeli M B, Mitra R S
出版信息
Appl Environ Microbiol. 1981 Jan;41(1):46-50. doi: 10.1128/aem.41.1.46-50.1981.
Abstract
An inducible cadmium-binding protein was isolated from Escherichia coli cells accommodated to 3 X 10(-6) M Cd2+ but not from normal or unaccommodated cells. Sephadex G-100, metal chelate affinity chromatography, and disc gel electrophoresis were used in the purification procedure. The molecular weight of the Cd2+-binding protein was estimated to be about 39,000 by Sephadex G-100 chromatography, making it different from the conventional, much smaller metallothionein.
摘要
一种可诱导的镉结合蛋白是从适应于3×10⁻⁶ M Cd²⁺ 的大肠杆菌细胞中分离得到的，而在正常或未适应的细胞中则未分离到。在纯化过程中使用了葡聚糖凝胶G - 100、金属螯合亲和层析和圆盘凝胶电泳。通过葡聚糖凝胶G - 100层析估计，镉结合蛋白的分子量约为39000，这使其不同于传统的、小得多的金属硫蛋白。
相似文献
1
Cadmium-binding component in Escherichia coli during accommodation to low levels of this ion.大肠杆菌在适应低水平镉离子过程中的镉结合成分。
Appl Environ Microbiol. 1981 Jan;41(1):46-50. doi: 10.1128/aem.41.1.46-50.1981.
2
Isolation and characterization of a low molecular weight cadmium-binding protein from Syrian hamster lung.叙利亚仓鼠肺中一种低分子量镉结合蛋白的分离与鉴定。
Toxicol Appl Pharmacol. 1980 Sep 15;55(2):370-7. doi: 10.1016/0041-008x(80)90099-x.
3
Purification of low molecular weight metal-binding proteins by preparative polyacrylamide gel electrophoresis: properties of electrophoretically purified rat liver (Cd, Zn) - metallothioneins.通过制备性聚丙烯酰胺凝胶电泳纯化低分子量金属结合蛋白：电泳纯化的大鼠肝脏（镉、锌）-金属硫蛋白的性质
Prep Biochem. 1980;10(4):495-505. doi: 10.1080/00327488008061746.
4
Purification and properties of plaice metallothionein, a cadmium-binding protein from the liver of the plaice (Pleuronectes platessa).鲽金属硫蛋白的纯化及特性，一种来自鲽（欧洲比目鱼）肝脏的镉结合蛋白。
Biochem J. 1979 Nov 1;183(2):277-83. doi: 10.1042/bj1830277.
5
Purification and molecular properties of two cadmium-binding glycoproteins from the hepatopancreas of a whelk, Buccinum tenuissimum.
Biochim Biophys Acta. 1983 May 30;745(1):50-60. doi: 10.1016/0167-4838(83)90169-3.
6
A high-molecular-weight cadmium-binding fraction isolated from the liver cytosol of trout exposed to environmentally relevant concentrations of the metal [proceedings].
Biochem Soc Trans. 1979 Aug;7(4):650-1. doi: 10.1042/bst0070650.
7
Isolation and properties of cadmium-binding protein induced in rat small intestine by oral administration of cadmium.
J Toxicol Environ Health. 1982 Mar;9(3):401-9. doi: 10.1080/15287398209530173.
8
Studies on metallothionein and cadmium.
Environ Res. 1978 Jun;15(3):381-404. doi: 10.1016/0013-9351(78)90120-2.
9
High-molecular-weight cadmium-binding proteins in rat liver cytosol: isolation and partial characterization of an approximately 50-kDa protein.大鼠肝细胞溶质中的高分子量镉结合蛋白：一种约50 kDa蛋白的分离与部分特性鉴定
J Biochem. 1987 Dec;102(6):1459-68. doi: 10.1093/oxfordjournals.jbchem.a122192.
10
Cadmium-binding proteins of rat testes. Characterization of a low-molecular-mass protein that lacks identity with metallothionein.大鼠睾丸中的镉结合蛋白。一种与金属硫蛋白无同源性的低分子量蛋白的特性。
Biochem J. 1984 Jun 15;220(3):811-8. doi: 10.1042/bj2200811.
引用本文的文献
1
Competition of Cd(II) and Pb(II) on the bacterial cells: a new insight from bioaccumulation based on NanoSIMS imaging.Cd(II) 和 Pb(II) 在细菌细胞上的竞争：基于 NanoSIMS 成像的生物累积的新见解。
Appl Environ Microbiol. 2024 Feb 21;90(2):e0145323. doi: 10.1128/aem.01453-23. Epub 2024 Jan 11.
2
Metal complexation by histidine-rich peptides confers protective roles against cadmium stress in as revealed by proteomics analysis.蛋白质组学分析表明，富含组氨酸的肽与金属络合赋予了对镉胁迫的保护作用。
PeerJ. 2018 Jul 26;6:e5245. doi: 10.7717/peerj.5245. eCollection 2018.
3
Mass balance of heavy metal uptake by encapsulated cultures ofKlebsiella aerogenes.好的，我已经了解任务，请你提供需要翻译的文本。
Microb Ecol. 1983 Oct;9(3):261-72. doi: 10.1007/BF02097741.
4
Extracellular sensors and extracellular alarmones, which permit cross-talk between organisms, determine the levels of alkali tolerance and trigger alkaliinduced acid sensitivity in Escherichia coli.细胞外传感器和细胞外警报素能够实现生物体之间的相互交流，它们决定了大肠杆菌的耐碱性水平，并引发碱诱导的酸敏感性。
Sci Prog. 2005;88(Pt 3):133-56. doi: 10.3184/003685005783238417.
5
Adaptation to Cadmium by Klebsiella aerogenes Growing in Continuous Culture Proceeds Mainly via Formation of Cadmium Sulfide.在连续培养中生长的产气克雷伯氏菌通过形成硫化镉来适应镉。
Appl Environ Microbiol. 1982 Oct;44(4):938-44. doi: 10.1128/aem.44.4.938-944.1982.
6
How killed enterobacterial cultures can activate living organisms to resist lethal agents or conditions.已杀灭的肠杆菌培养物如何激活生物体以抵抗致死因子或条件。
Sci Prog. 2003;86(Pt 3):157-78. doi: 10.3184/003685003783238662.
7
Extracellular proteins as enterobacterial thermometers.作为肠道细菌温度计的细胞外蛋白质。
Sci Prog. 2003;86(Pt 1-2):139-55. doi: 10.3184/003685003783238716.
8
Extracellular sensing and signalling pheromones switch-on thermotolerance and other stress responses in Escherichia coli.细胞外传感和信号信息素开启大肠杆菌的耐热性及其他应激反应。
Sci Prog. 2001;84(Pt 3):205-33. doi: 10.3184/003685001783238970.
9
Inorganic phosphate accumulation and cadmium detoxification in Klebsiella aerogenes NCTC 418 growing in continuous culture.在连续培养条件下生长的产气克雷伯菌NCTC 418中的无机磷酸盐积累和镉解毒作用。
Appl Environ Microbiol. 1984 Feb;47(2):374-7. doi: 10.1128/aem.47.2.374-377.1984.
10
Interrelationships in trace-element metabolism in metal toxicities in nickel-resistant strains of Neurospora crassa.粗糙脉孢菌镍抗性菌株中金属毒性下微量元素代谢的相互关系。
Biochem J. 1983 Apr 15;212(1):205-10. doi: 10.1042/bj2120205.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
3
Cadmium and zinc in pregnancy and lactation.孕期及哺乳期的镉与锌。
Arch Environ Health. 1972 Jul;25(1):14-22. doi: 10.1080/00039896.1972.10666127.
4
Cadmium and zinc binding in mammalian liver and kidneys.镉和锌在哺乳动物肝脏和肾脏中的结合
Arch Environ Health. 1972 Jun;24(6):419-25. doi: 10.1080/00039896.1972.10666118.
5
Estimation of the molecular weights of proteins by Sephadex gel-filtration.用葡聚糖凝胶过滤法估算蛋白质的分子量
Biochem J. 1964 May;91(2):222-33. doi: 10.1042/bj0910222.
6
Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli.大肠杆菌细胞壁和细胞质膜的蛋白质组成。
J Bacteriol. 1970 Nov;104(2):890-901. doi: 10.1128/jb.104.2.890-901.1970.
7
Relative cadmium-binding capacity of metallothionein and other cytosolic fractions in various tissues of the rat.大鼠不同组织中金属硫蛋白及其他胞质组分的相对镉结合能力。
Environ Physiol Biochem. 1975;5(6):378-88.
8
Molecular mechanisms of accommodation in Escherichia coli to toxic levels of Cd2+.大肠杆菌对Cd2+毒性水平的适应性分子机制。
J Bacteriol. 1975 Mar;121(3):1180-8. doi: 10.1128/jb.121.3.1180-1188.1975.
9
Amino-acid sequence of equine renal metallothionein-1B.马肾金属硫蛋白-1B的氨基酸序列。
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3413-7. doi: 10.1073/pnas.73.10.3413.
10
Turnover of metallothioneins in rat liver.大鼠肝脏中金属硫蛋白的周转
Biochem J. 1978 Jul 15;174(1):327-38. doi: 10.1042/bj1740327.
Zotero 插件