Abbott I A, Chapman F A
Arch Microbiol. 1981 Feb;128(4):355-9. doi: 10.1007/BF00405912.
Seventy-one samples of the colloid kappa-carrageenan extracted from 12 seaweed species were subjected to a number of standard physical demands of solid bacteriological culture media. All samples had a lower melting temperature (less than 67 degrees C) than agar and a gelling (setting) temperature between l6 degrees C and 51 degrees C, some the same and others lower or higher than agar. Temperature spreads were narrow (ca 10 degrees C) to broad (ca 30 degrees C), depending on the seaweed source, but none were as broad as that of agar (ca 40 degrees C). The majority of commercially prepared samples held a slant when incubated at 37 degrees C, but California seaweed colloids were best at 28 degrees C in this test. The majority of samples released little to no water of syneresis in slant tests as well as in plates. Some plates prepared with the colloid were crystal clear as compared to agar plates. All test microorganisms grew as well on kappa-carrageenan media s on agar media. Some media responses could be attributable to the seaweed species, but others could be traced to chemical extraction methods and modification of the colloid.
从12种海藻中提取的71份胶体κ-卡拉胶样品,接受了固体细菌培养基的多项标准物理要求测试。所有样品的熔点温度(低于67摄氏度)均低于琼脂,凝胶(凝固)温度在16摄氏度至51摄氏度之间,有些与琼脂相同,有些则低于或高于琼脂。温度范围窄(约10摄氏度)至宽(约30摄氏度),取决于海藻来源,但均不如琼脂宽(约40摄氏度)。大多数商业制备的样品在37摄氏度培养时呈斜面状,但在此测试中,加利福尼亚海藻胶体在28摄氏度时表现最佳。大多数样品在斜面测试和平板测试中几乎不产生或不产生脱水收缩水。与琼脂平板相比,一些用该胶体制备的平板清澈透明。所有测试微生物在κ-卡拉胶培养基上的生长情况与在琼脂培养基上一样好。一些培养基反应可能归因于海藻种类,但其他反应可追溯到化学提取方法和胶体的改性。