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κ-卡拉胶作为微生物培养基中琼脂替代品的评估。

Evaluation of kappa carrageenan as a substitute for agar in microbiological media.

作者信息

Abbott I A, Chapman F A

出版信息

Arch Microbiol. 1981 Feb;128(4):355-9. doi: 10.1007/BF00405912.

DOI:10.1007/BF00405912
PMID:7013726
Abstract

Seventy-one samples of the colloid kappa-carrageenan extracted from 12 seaweed species were subjected to a number of standard physical demands of solid bacteriological culture media. All samples had a lower melting temperature (less than 67 degrees C) than agar and a gelling (setting) temperature between l6 degrees C and 51 degrees C, some the same and others lower or higher than agar. Temperature spreads were narrow (ca 10 degrees C) to broad (ca 30 degrees C), depending on the seaweed source, but none were as broad as that of agar (ca 40 degrees C). The majority of commercially prepared samples held a slant when incubated at 37 degrees C, but California seaweed colloids were best at 28 degrees C in this test. The majority of samples released little to no water of syneresis in slant tests as well as in plates. Some plates prepared with the colloid were crystal clear as compared to agar plates. All test microorganisms grew as well on kappa-carrageenan media s on agar media. Some media responses could be attributable to the seaweed species, but others could be traced to chemical extraction methods and modification of the colloid.

摘要

从12种海藻中提取的71份胶体κ-卡拉胶样品,接受了固体细菌培养基的多项标准物理要求测试。所有样品的熔点温度(低于67摄氏度)均低于琼脂,凝胶(凝固)温度在16摄氏度至51摄氏度之间,有些与琼脂相同,有些则低于或高于琼脂。温度范围窄(约10摄氏度)至宽(约30摄氏度),取决于海藻来源,但均不如琼脂宽(约40摄氏度)。大多数商业制备的样品在37摄氏度培养时呈斜面状,但在此测试中,加利福尼亚海藻胶体在28摄氏度时表现最佳。大多数样品在斜面测试和平板测试中几乎不产生或不产生脱水收缩水。与琼脂平板相比,一些用该胶体制备的平板清澈透明。所有测试微生物在κ-卡拉胶培养基上的生长情况与在琼脂培养基上一样好。一些培养基反应可能归因于海藻种类,但其他反应可追溯到化学提取方法和胶体的改性。

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本文引用的文献

1
Substitute for agar in solid media for common usages in microbiology.用于微生物学常见用途的固体培养基中琼脂的替代品。
Appl Environ Microbiol. 1976 Apr;31(4):509-13. doi: 10.1128/aem.31.4.509-513.1976.
2
Value of the K+ salt of carageenan as an agar substitute in routine bacteriological media.角叉菜胶钾盐作为常规细菌学培养基中琼脂替代品的价值。
Appl Environ Microbiol. 1977 Dec;34(6):637-9. doi: 10.1128/aem.34.6.637-639.1977.
磷酸盐催化琼脂、结冷胶和κ-卡拉胶生成过氧化氢以及通过过氧化氢酶和丙酮酸恢复微生物可培养性
Appl Environ Microbiol. 2017 Oct 17;83(21). doi: 10.1128/AEM.01366-17. Print 2017 Nov 1.
4
Progress in the development of gelling agents for improved culturability of microorganisms.用于提高微生物可培养性的凝胶剂的开发进展。
Front Microbiol. 2015 Jul 23;6:698. doi: 10.3389/fmicb.2015.00698. eCollection 2015.