Laughrea M
Biochimie. 1981 Mar;63(3):145-68. doi: 10.1016/s0300-9084(81)80189-7.
Poly U-directed incorporation of phenylalanine and leucine into polypeptide has been described in at least 50 papers since 1961. In general, high translation activities are associated with high accuracies, and vice-versa. Moreover, a vast body of independent experimental data (effect of ethanol, temperature, urea, aminoglycosides, etc... on protein synthesis) put together here suggests that, in many circumstances, speed and accuracy of elongation are correlated. This result is to be contrasted with the view that the speed and the fidelity of protein synthesis are two opposing parameters. In this report, recent experimental data on the nature and effect of ribosomal ambiguity (ram) and streptomycin resistance (Strr) mutations are reexamined. Models on the action of streptomycin and other misreading-inducing antibiotics, as well as long-standing ideas on the control of misreading in mammalian systems are critically evaluated. An explanation is provided for the long-befuddling data on the action of gentamicin.
自1961年以来，至少有50篇论文描述了在多聚尿嘧啶指导下将苯丙氨酸和亮氨酸掺入多肽的过程。一般来说，高翻译活性与高准确性相关，反之亦然。此外，这里汇总的大量独立实验数据（乙醇、温度、尿素、氨基糖苷类等对蛋白质合成的影响）表明，在许多情况下，延伸的速度和准确性是相关的。这一结果与蛋白质合成的速度和保真度是两个相互对立参数的观点形成对比。在本报告中，重新审视了关于核糖体模糊性（ram）和链霉素抗性（Strr）突变的性质和影响的最新实验数据。对链霉素和其他诱导错读的抗生素的作用模型，以及关于哺乳动物系统中错读控制的长期观点进行了批判性评估。对庆大霉素作用的长期令人困惑的数据提供了一种解释。
