Kirsebom L A, Isaksson L A
Proc Natl Acad Sci U S A. 1985 Feb;82(3):717-21. doi: 10.1073/pnas.82.3.717.
The effects of two mutations, which map at the rplL locus and both give a changed 50S ribosomal protein L7/L12, were studied. Both mutations are associated with an increased misreading of all three nonsense codons in vivo and ribosomes from the mutants give an increased misreading of the phenylalanine codon UUU by tRNALeu in vitro. The rplL-associated misreading in vitro is not limited to a particular type of mRNA or tRNA. Results from a translational proofreading assay, using mutant ribosomes, suggest that protein L7/L12 is involved in the control of translational accuracy by contributing to the efficiency of a translational proofreading step(s).
研究了位于rplL位点的两个突变的影响，这两个突变均导致50S核糖体蛋白L7/L12发生改变。这两个突变都与体内所有三种无义密码子的错读增加有关，并且来自突变体的核糖体在体外使亮氨酰tRNA对苯丙氨酸密码子UUU的错读增加。体外与rplL相关的错读并不局限于特定类型的mRNA或tRNA。使用突变核糖体进行的翻译校对试验结果表明，蛋白L7/L12通过促进翻译校对步骤的效率参与翻译准确性的控制。
